Colistin resistance gene mcr-1 in gut flora of children
| Autor: | Zi-Xian Huang, Hanyu Wang, Rong Zhang, Yanyan Hu, Qiaoling Sun, Yue-ling Wang, Gong-Xiang Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Gene Transfer Horizontal medicine.drug_class Polymyxin 030106 microbiology Microbial Sensitivity Tests Quinolones medicine.disease_cause beta-Lactamases Microbiology Feces 03 medical and health sciences Drug Resistance Bacterial Escherichia coli Pulsed-field gel electrophoresis medicine Humans Pharmacology (medical) Polymyxins Child biology Colistin Escherichia coli Proteins General Medicine biology.organism_classification Antimicrobial Enterobacteriaceae Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Gastrointestinal Microbiome Citrobacter freundii Infectious Diseases Multilocus sequence typing MCR-1 hormones hormone substitutes and hormone antagonists Multilocus Sequence Typing Plasmids |
| Zdroj: | International Journal of Antimicrobial Agents. 50:593-597 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2017.06.011 |
| Popis: | The aim of this study was to determine the prevalence and transmission mechanism(s) of mcr-1 in the gut flora of children. Faecal samples (n = 173) were obtained from non-diarrhoea patients at the Children's Hospital of Zhejiang University (Hangzhou, China). PCR-based analysis indicated that 17 isolates from 9.8% of the samples were positive for mcr-1, comprising 16 Escherichia coli and 1 Citrobacter freundii. Nine mcr-1-bearing isolates co-expressed extended-spectrum β-lactamase (ESBL) genes, but plasmid-mediated quinolone resistance (PMQR) genes were not detected. Transconjugation followed by Southern hybridisation analysis revealed that 14 of the E. coli isolates were able to transfer their colistin-resistant phenotype to E. coli EC600. All 14 of these E. coli strains contained a major mcr-1-containing conjugative plasmid with a size of ca. 33 kb or 55 kb. All but two of the E. coli isolates presented distinct pulsed-field gel electrophoresis (PFGE) patterns. Multilocus sequence typing (MLST) analysis revealed 11 sequence types (STs) among the E. coli 16 isolates, with ST117 being the most common. The finding of a high prevalence of mcr-1 in the intestinal flora of children, with the majority of mcr-1-positive isolates being E. coli, highlights the need for more rational use of polymyxins to prevent polymyxin resistance from becoming disseminated among different microbial pathogens. Given the high detection rate of mcr-1 in children, we recommend that polymyxin is no longer used as a last-resort antimicrobial agent and that alternative strategies are developed to treat infections caused by such pathogens. |
| Databáze: | OpenAIRE |
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