Development of a PCR test based on a gene region associated with the pathogenicity of Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia in Asia
| Autor: | L.M. Thomas, S.K. Brickell, K.A. Long, P.R. Widders, Michael Panaccio |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Serotype Pasteurella multocida Buffaloes Molecular Sequence Data Cattle Diseases Enzyme-Linked Immunosorbent Assay Hemorrhagic septicemia Bacterial genome size Biology Polymerase Chain Reaction Microbiology law.invention law RNA Ribosomal 16S medicine Animals Serotyping Hemorrhagic Septicemia Polymerase chain reaction DNA Primers Electrophoresis Agar Gel Base Sequence General Veterinary Nucleic acid sequence General Medicine Ribosomal RNA biology.organism_classification medicine.disease Virology Blotting Southern RNA Ribosomal 23S Cattle Pasteurellosis |
| Zdroj: | Veterinary Microbiology. 59:295-307 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(97)00199-5 |
| Popis: | We have developed a PCR assay to detect Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia (HS) in Asia. Nucleotide sequence determination of a 16S rRNA-23S rRNA PCR product unique to B:2 strains was shown to share amino acid sequence homology with a bacteriophage Mu protein. Primers designed from this sequence when tested against a panel of isolates recovered from a wide geographical area and representing a large range of bacterial genera and species, were found to specifically amplify DNA from P. multocida, serotype B:2. Southern hybridisation confirmed the presence of this sequence in only the B:2 serotype of P. multocida, suggesting an association between bacterial virulence and the presence of bacteriophage genes in the bacterial genome. The results of this study demonstrate the potential application of PCR to the diagnosis of HS in cattle and buffalo in Asia. Application of PCR to support diagnosis of HS will greatly improve accuracy, laboratory response time, and will facilitate rational deployment of resources for controlling this disease. |
| Databáze: | OpenAIRE |
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