Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL
| Autor: | Noraini Mahmad, Bakrudeen Ali Ahmed Abdul, Sadegh Mohajer, Rosna Mat Taha, Sharifah Nurashikin Wafa |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pathology medicine.medical_specialty Article Subject Organogenesis Cell Culture Techniques Zingiberales lcsh:Medicine Plant Roots 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Murashige and Skoog medium In vivo medicine Canna indica Plants Medicinal General Immunology and Microbiology biology lcsh:R fungi food and beverages General Medicine biology.organism_classification Rhizome Plant Leaves Horticulture 030104 developmental biology Micropropagation Seedlings Shoot Plant Shoots Research Article 010606 plant biology & botany Explant culture |
| Zdroj: | BioMed Research International, Vol 2016 (2016) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2016/2820454 |
| Popis: | An efficient protocol for micropropagation ofCanna indicaL., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks. Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%). Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival. In addition, ultrastructural studies showed that rhizomes ofin vitrogrown specimens were underdeveloped compared to thein vivospecimens, possibly due to the presence of wide spaces. Meanwhile, the leaves ofin vivospecimens had more open stomata compared toin vitrospecimens, yet their paracytic stomata structures were similar. Hence, there were no abnormalities or major differences betweenin vitroregenerants and mother plants. |
| Databáze: | OpenAIRE |
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