Controlled Transcription of Regulator Gene carS by Tet-on or by a Strong Promoter Confirms Its Role as a Repressor of Carotenoid Biosynthesis in Fusarium fujikuroi
| Autor: | Marente Bernal, Julia Natividad, Ávalos Cordero, Francisco Javier, Limón Mirón, María del Carmen, Francisco Javier Ávalos Cordero |
|---|---|
| Přispěvatelé: | Universidad de Sevilla. Departamento de Genética, Ministerio de Economía y Competitividad (MINECO). España, Ministerio de Ciencia e Innovación (MICIN). España, Agencia Estatal de Investigación. España, European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Tet-on Mutant Fusarium fujikuroi carS Repressor Microbiology Article 03 medical and health sciences Downregulation and upregulation Transcription (biology) Virology Ring finger medicine lcsh:QH301-705.5 Gene 030304 developmental biology Regulator gene 0303 health sciences 030306 microbiology Chemistry Structural gene carotenoids luciferase mluc reporter gene Cell biology medicine.anatomical_structure lcsh:Biology (General) human activities |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) Microorganisms, Vol 9, Iss 71, p 71 (2021) Microorganisms Volume 9 Issue 1 |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms9010071 |
| Popis: | Carotenoid biosynthesis is a frequent trait in fungi. In the ascomycete Fusarium fujikuroi, the synthesis of the carboxylic xanthophyll neurosporaxanthin (NX) is stimulated by light. However, the mutants of the carS gene, encoding a protein of the RING finger family, accumulate large NX amounts regardless of illumination, indicating the role of CarS as a negative regulator. To confirm CarS function, we used the Tet-on system to control carS expression in this fungus. The system was first set up with a reporter mluc gene, which showed a positive correlation between the inducer doxycycline and luminescence. Once the system was improved, the carS gene was expressed using Tet-on in the wild strain and in a carS mutant. In both cases, increased carS transcription provoked a downregulation of the structural genes of the pathway and albino phenotypes even under light. Similarly, when the carS gene was constitutively overexpressed under the control of a gpdA promoter, total downregulation of the NX pathway was observed. The results confirmed the role of CarS as a repressor of carotenogenesis in F. fujikuroi and revealed that its expression must be regulated in the wild strain to allow appropriate NX biosynthesis in response to illumination. Ministerio de Economía y Competitividad del Gobierno de España-BIO2015-69613-R Ministerio de Ciencia e Innovación (MCI), Agencia Estatal de Investigación (AEI); de España y Fondo Europeo de Desarrollo Regional (FEDER)-RTI2018-101902-B-I00 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|