Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’ by Z. Shen, A. Webster, K. J. Welham, C. E. Dyer, J. Greenman and S. J. Haswell
| Autor: | Bibiana Beckmann, Jörg Sandmann, Dimitrios Tsikas |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Flow injection analysis
Spectrometry Mass Electrospray Ionization Benzotriazole Electrospray ionization Analytical chemistry Triazole Protonation Phenylenediamines Nitric Oxide Mass spectrometry Biochemistry Analytical Chemistry Nitric oxide Intestines chemistry.chemical_compound chemistry Electrochemistry Animals Environmental Chemistry Intestinal Mucosa Hypoxia Aorta Spectroscopy Derivative (chemistry) Nuclear chemistry |
| Zdroj: | The Analyst. 136:407-410 |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | Recently, Shen et al. (Analyst, 2010, 135, 302) reported on a flow injection analysis (FIA) ESI-MS/MS approach for the determination of the short-lived gaseous nitric oxide (NO) in biological samples. This method is based on the reaction of NO, and presumably of other NO-derived oxides such as N(2)O(3), with the vicinal amino groups of methylpiperazinobenzendiamine to form a benzotriazole derivative. Under MS/MS conditions, the protonated derivative loses molecular nitrogen (N(2)) from the triazole ring and the product ion formed is utilized for quantitative analyses. This seems to be the first ESI-MS/MS method for authentic NO detection and quantification. However, the ESI-MS/MS method reported by Shen et al. deserves some critical discussion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|