Procyanidins Mitigate Osteoarthritis Pathogenesis by, at Least in Part, Suppressing Vascular Endothelial Growth Factor Signaling
Autor: | Neil J. Cobelli, Angela Wang, Sun Jin Kim, Lidi Liu, Hui B. Sun, David M. Hirsh, Lin Xu, Zhiyong He, John A. Hardin, Daniel J. Leong |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Pharmacology Catalysis Inorganic Chemistry Pathogenesis lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound chondroprotection Gene expression post-traumatic osteoarthritis Procyanidin B3 Physical and Theoretical Chemistry Molecular Biology Procyanidin B2 lcsh:QH301-705.5 Spectroscopy nutraceuticals Organic Chemistry Kinase insert domain receptor General Medicine procyanidins osteoarthritis VEGF pine bark extract Computer Science Applications Vascular endothelial growth factor Vascular endothelial growth factor A 030104 developmental biology chemistry Biochemistry lcsh:Biology (General) lcsh:QD1-999 Phosphorylation |
Zdroj: | International Journal of Molecular Sciences; Volume 17; Issue 12; Pages: 2065 International Journal of Molecular Sciences, Vol 17, Iss 12, p 2065 (2016) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms17122065 |
Popis: | Procyanidins are a family of plant metabolites that have been suggested to mitigate osteoarthritis pathogenesis in mice. However, the underlying mechanism is largely unknown. This study aimed to determine whether procyanidins mitigate traumatic injury-induced osteoarthritis (OA) disease progression, and whether procyanidins exert a chondroprotective effect by, at least in part, suppressing vascular endothelial growth factor signaling. Procyanidins (extracts from pine bark), orally administered to mice subjected to surgery for destabilization of the medial meniscus, significantly slowed OA disease progression. Real-time polymerase chain reaction revealed that procyanidin treatment reduced expression of vascular endothelial growth factor and effectors in OA pathogenesis that are regulated by vascular endothelial growth factor. Procyanidin-suppressed vascular endothelial growth factor expression was correlated with reduced phosphorylation of vascular endothelial growth factor receptor 2 in human OA primary chondrocytes. Moreover, components of procyanidins, procyanidin B2 and procyanidin B3 exerted effects similar to those of total procyanidins in mitigating the OA-related gene expression profile in the primary culture of human OA chondrocytes in the presence of vascular endothelial growth factor. Together, these findings suggest procyanidins mitigate OA pathogenesis, which is mediated, at least in part, by suppressing vascular endothelial growth factor signaling. |
Databáze: | OpenAIRE |
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