Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori
Autor: | Qiwei Sun, Jingwen Ma, Yao Ou, Yan Ma, Lihua Huang, Hanfu Xu, Qin Luo, Wenhui Zeng |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Life Cycles
Microarrays genetic processes Gene Expression Protein Synthesis Genome Biochemistry Larvae Animal Products Gene expression Protein biosynthesis Phylogeny Data Management Multidisciplinary Chemical Synthesis Eukaryota Agriculture Phylogenetic Analysis Phylogenetics Insects Bioassays and Physiological Analysis Medicine DNA microarray Research Article Computer and Information Sciences Biosynthetic Techniques Arthropoda Science Silk Computational biology Biology Research and Analysis Methods Bombyx mori Genetics Animals Evolutionary Systematics Gene Regulation natural sciences Transcription factor Silk gland Taxonomy Evolutionary Biology fungi Organisms Biology and Life Sciences Proteins biology.organism_classification Bombyx Invertebrates Silkworms Homeobox Transcriptome Zoology Entomology Developmental Biology Transcription Factors |
Zdroj: | PLoS ONE, Vol 16, Iss 11 (2021) PLoS ONE, Vol 16, Iss 11, p e0259870 (2021) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Transcription factors (TFs) are key proteins that modulate gene transcription and thereby lead to changes in the gene expression profile and the subsequent alteration of cellular functions. In the silk gland (SG) of silkworm Bombyx mori, an important silk-producing insect, TFs are of vital importance in the regulation of silk protein synthesis in this organ. However, which TFs exist and express in the SG remains largely unknown. Here, we report the large-scale identification of TFs in the SG based on available full-length transcript sequences and the most recent version of silkworm genome data. In total, 348 candidate TFs were identified by strict filtration and were classified into 56 TF families. Chromosomal distribution, motif composition, and phylogenetic relationship analyses revealed the typical characteristics of these TFs. In addition, the expression patterns of 348 TFs in various tissues of B. mori, especially the SG of fourth-molt (4LM) and day-3 and day-4 fifth-instar (5L3D and 5L4D) larvae, were investigated based on public RNA-seq data and gene microarray data, followed by spatiotemporal verification of TF expression levels by quantitative real-time PCR (qRT-PCR). This report describes the first comprehensive analysis of TFs in the B. mori SG. The results can serve as a baseline for further studies of the roles of TFs in the B. mori SG. |
Databáze: | OpenAIRE |
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