Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori

Autor: Qiwei Sun, Jingwen Ma, Yao Ou, Yan Ma, Lihua Huang, Hanfu Xu, Qin Luo, Wenhui Zeng
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Life Cycles
Microarrays
genetic processes
Gene Expression
Protein Synthesis
Genome
Biochemistry
Larvae
Animal Products
Gene expression
Protein biosynthesis
Phylogeny
Data Management
Multidisciplinary
Chemical Synthesis
Eukaryota
Agriculture
Phylogenetic Analysis
Phylogenetics
Insects
Bioassays and Physiological Analysis
Medicine
DNA microarray
Research Article
Computer and Information Sciences
Biosynthetic Techniques
Arthropoda
Science
Silk
Computational biology
Biology
Research and Analysis Methods
Bombyx mori
Genetics
Animals
Evolutionary Systematics
Gene Regulation
natural sciences
Transcription factor
Silk gland
Taxonomy
Evolutionary Biology
fungi
Organisms
Biology and Life Sciences
Proteins
biology.organism_classification
Bombyx
Invertebrates
Silkworms
Homeobox
Transcriptome
Zoology
Entomology
Developmental Biology
Transcription Factors
Zdroj: PLoS ONE, Vol 16, Iss 11 (2021)
PLoS ONE, Vol 16, Iss 11, p e0259870 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Transcription factors (TFs) are key proteins that modulate gene transcription and thereby lead to changes in the gene expression profile and the subsequent alteration of cellular functions. In the silk gland (SG) of silkworm Bombyx mori, an important silk-producing insect, TFs are of vital importance in the regulation of silk protein synthesis in this organ. However, which TFs exist and express in the SG remains largely unknown. Here, we report the large-scale identification of TFs in the SG based on available full-length transcript sequences and the most recent version of silkworm genome data. In total, 348 candidate TFs were identified by strict filtration and were classified into 56 TF families. Chromosomal distribution, motif composition, and phylogenetic relationship analyses revealed the typical characteristics of these TFs. In addition, the expression patterns of 348 TFs in various tissues of B. mori, especially the SG of fourth-molt (4LM) and day-3 and day-4 fifth-instar (5L3D and 5L4D) larvae, were investigated based on public RNA-seq data and gene microarray data, followed by spatiotemporal verification of TF expression levels by quantitative real-time PCR (qRT-PCR). This report describes the first comprehensive analysis of TFs in the B. mori SG. The results can serve as a baseline for further studies of the roles of TFs in the B. mori SG.
Databáze: OpenAIRE
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