Serum Metabolomic Profiles of Paratuberculosis Infected and Infectious Dairy Cattle by Ambient Mass Spectrometry
| Autor: | Paola Fiorini, Jeroen De Buck, Annalisa Stefani, Massimo Bottazzari, Mauro Dal Prà, Roberto Piro, Brunella Miano, Andrea Massaro, Laura Paganini, Ivana Pallante, Alessandra Tata, Nicola Pozzato |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
DART-MS Paratuberculosis Biology Creatine 01 natural sciences Microbiology Incubation period 03 medical and health sciences chemistry.chemical_compound medicine Dairy cattle Feces Original Research Subclinical infection Creatinine lcsh:Veterinary medicine General Veterinary 010401 analytical chemistry Jonhe's disease medicine.disease 0104 chemical sciences paratuberculosis 030104 developmental biology chemistry cattle Herd lcsh:SF600-1100 biomarker Veterinary Science mycobacterium avium subsp paratubercolosis metabolomic |
| Zdroj: | Frontiers in Veterinary Science Frontiers in Veterinary Science, Vol 7 (2021) |
| ISSN: | 2297-1769 |
| Popis: | Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis [Johne's disease (JD)], a chronic disease that causes substantial economic losses in the dairy cattle industry. The long incubation period means clinical signs are visible in animals only after years, and some cases remain undetected because of the subclinical manifestation of the disease. Considering the complexity of JD pathogenesis, animals can be classified as infected, infectious, or affected. The major limitation of currently available diagnostic tests is their failure in detecting infected non-infectious animals. The present study aimed to identify metabolic markers associated with infected and infectious stages of JD. Direct analysis in real time coupled with high resolution mass spectrometry (DART-HRMS) was, hence, applied in a prospective study where cohorts of heifers and cows were followed up annually for 2–4 years. The animals' infectious status was assigned based on a positive result of both serum ELISA and fecal PCR, or culture. The same animals were retrospectively assigned to the status of infected at the previous sampling for which all JD tests were negative. Stored sera from 10 infected animals and 17 infectious animals were compared with sera from 20 negative animals from the same herds. Two extraction protocols and two (-/+) ionization modes were tested. The three most informative datasets out of the four were merged by a mid-level data fusion approach and submitted to partial least squares discriminant analysis (PLS-DA). Compared to the MAP negative subjects, metabolomic analysis revealed the m/z signals of isobutyrate, dimethylethanolamine, palmitic acid, and rhamnitol were more intense in infected animals. Both infected and infectious animals showed higher relative intensities of tryptamine and creatine/creatinine as well as lower relative abundances of urea, glutamic acid and/or pyroglutamic acid. These metabolic differences could indicate altered fat metabolism and reduced energy intake in both infected and infectious cattle. In conclusion, DART-HRMS coupled to a mid-level data fusion approach allowed the molecular features that identified preclinical stages of JD to be teased out. |
| Databáze: | OpenAIRE |
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