Two-Photon Probe for TNF-α. Assessment of the Transmembrane TNF-α Level in Human Colon Tissue by Two-Photon Microscopy
| Autor: | Hoon Jai Chun, Man So Han, Kyu Cheol Paik, Mun Seok Kim, Jung Nyoung Heo, Ji Woo Choi, Min Cheol Pyo, Eun Jeong Lee, Bong Rae Cho, Eun Sun Kim, Seung Taek Hong, Byumseok Koh, Eunyoung Yoon, Seong Ji Choi |
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| Rok vydání: | 2019 |
| Předmět: |
Photolysis
Chemistry Cell Survival Colon Tumor Necrosis Factor-alpha Analytical chemistry Carbazoles Hydrogen-Ion Concentration Transmembrane protein Infliximab Analytical Chemistry Mice Microscopy Fluorescence Multiphoton RAW 264.7 Cells Two-photon excitation microscopy Aqueous buffer Animals Humans Absorption (electromagnetic radiation) Human colon Fluorescent Dyes |
| Zdroj: | Analytical chemistry. 91(24) |
| ISSN: | 1520-6882 |
| Popis: | We developed Pyr1-infliximab: a two-photon probe for TNF-α. Pyr1-infliximab showed absorption maxima at 280 and 438 nm and an emission maximum at 610 nm in an aqueous buffer and effective two-photon action cross-section values of (520-2830) × 10 |
| Databáze: | OpenAIRE |
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