Generation of a tropoelastin mRNA variant by alternative polyadenylation site selection in sun-damaged human skin and ultraviolet B-irradiated fibroblasts
| Autor: | Joel M. Gelfand, Lorraine H. Kligman, Elaine Schwartz, James C. Mauch |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Ultraviolet Rays Biophysics Human skin In Vitro Techniques Biochemistry Polymerase Chain Reaction Polyadenylation site Mice Tropoelastin Animals Humans RNA Messenger RNA Processing Post-Transcriptional Molecular Biology Aged DNA Primers Skin Messenger RNA Mice Hairless Binding Sites integumentary system biology Base Sequence Chemistry Genetic Variation Ultraviolet b Cell Biology Fibroblasts Molecular biology In vitro Hairless biology.protein Sunlight Female Elastin |
| Zdroj: | Biochemical and biophysical research communications. 246(1) |
| ISSN: | 0006-291X |
| Popis: | The goal of this research was to delineate the posttranscriptional mechanisms responsible for the increased elastin synthesis characteristic of sundamaged skin. In this study, a unique molecular variant of the tropoelastin mRNA transcript was identified in human sundamaged skin that was derived from the usage of an alternate polyadenylation site. Nonsolar exposed human skin expressed one tropoelastin mRNA species whereas sundamaged human skin expressed the primary tropoelastin mRNA and a larger, alternate tropoelastin mRNA formed from the utilization of a second polyadenylation site. Cultured human skin fibroblasts expressed both tropoelastin transcripts and in vitro UV treatment increased the amount of the unique tropoelastin mRNA. Hairless mouse skin (normal and UV treated) expressed the primary tropoelastin transcript although UV irradiation increased the length of its poly (A) tail two-fold. Therefore, UV radiation may stimulate elastin production by affecting polyadenylation site selection and the poly (A) tail length of tropoelastin mRNA. |
| Databáze: | OpenAIRE |
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