Metabolic imbalance of the insulin-like growth factor–I axis in Zucker diabetic fatty rats
| Autor: | Frédéric De Ceuninck, Audrey Caliez, Jean-Olivier Rolin, Louise Baschet, Alain Ktorza |
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| Rok vydání: | 2011 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism medicine.medical_treatment Diabetes Mellitus Experimental Insulin-like growth factor chemistry.chemical_compound Endocrinology Metabolic Diseases Corticosterone Diabetes mellitus Internal medicine medicine Animals Glucose homeostasis Obesity Insulin-Like Growth Factor I Principal Component Analysis Growth factor Insulin nutritional and metabolic diseases Type 2 Diabetes Mellitus medicine.disease Rats Rats Zucker Insulin-Like Growth Factor Binding Proteins Postprandial chemistry Metabolic Networks and Pathways |
| Zdroj: | Metabolism. 60:1575-1583 |
| ISSN: | 0026-0495 |
| Popis: | In healthy conditions, insulin-like growth factor-I (IGF-I) acts in a coordinated fashion with insulin to lower glycemia, mainly by increasing insulin sensitivity in peripheral tissues. The aim of this study was to explore the relationship between glucose homeostasis and the endocrine IGF-I axis in Zucker diabetic fatty (ZDF) rats. The plasma levels of glucose, insulin, growth hormone, free IGF-I, total IGF-I (associated to insulin-like growth factor binding proteins plus free), and corticosterone were measured in 13-week-old ZDF rats and in age-matched controls under fasting and postprandial conditions. The plasma IGF-I binding capacity was measured by radioligand binding. In ZDF rats, fasting total and free IGF-I levels were reduced by 22% and 92%, respectively, compared with controls. Postprandial free IGF-I was reduced by 35%, whereas total IGF-I was unaffected. The plasma IGF-I binding capacity in ZDF rats was reduced by 24% after fasting and by 13% under postprandial conditions. A clear correlation between free IGF-I and insulin was observed in postprandial controls but not in ZDF rats. A principal component analysis clearly separated ZDF and control rats into 2 main components under both fasting and postprandial conditions. The first component was determined equally by total IGF-I, bound IGF-I, the free to total IGF-I ratio, and the IGF-I binding capacity. The second component was determined mostly by glucose and insulin. Our results show a marked alteration of the plasma IGF-I levels and of the capacity of plasma to bind IGF-I, and a disturbed relationship between IGF-I and postprandial insulinemia in a rat model of type 2 diabetes mellitus. |
| Databáze: | OpenAIRE |
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