Microbial film formation: dental plaque deposition on acrylic tiles using continuous culture techniques
| Autor: | A. B. Dowsett, C. W. Keevil, D. J. Bradshaw, T. W. Feary |
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| Rok vydání: | 1987 |
| Předmět: |
Adult
Dental Plaque Chemostat Dental plaque Bacterial Physiological Phenomena Applied Microbiology and Biotechnology Microbiology Models Biological Bacterial Adhesion chemistry.chemical_compound Dental Materials medicine Humans Methylmethacrylates Food science Deposition (law) Growth medium Gram-Negative Anaerobic Bacteria biology Bacteria Biofilm Streptococcus biology.organism_classification medicine.disease Culture Media chemistry Rough surface Microscopy Electron Scanning Neisseria Gingival margin |
| Zdroj: | The Journal of applied bacteriology. 62(2) |
| ISSN: | 0021-8847 |
| Popis: | A chemostat system has been developed to model the attachment of oral bacteria, and the subsequent development of plaque film, to acrylic surfaces immersed in steady state cultures. Plaque was removed from the teeth and gingival margin of volunteers who refrained from oral hygiene for at least 72 h. Samples were pooled and inoculated into a complex growth medium maintained at 37 degrees C. Glucose-limited continuous culture was established at a dilution rate of 0.05/h and at pH 7.0. Microbiological analysis of the culture indicated that a complex community of oral bacteria was established, typical of that found in dental plaque. Acrylic tiles were immersed in the fermenter through a modified fermenter head and incubated therein for up to 21 d. Scanning electron microscopy showed that either side of the tiles contained a rough and a smooth surface and these initially favoured the attachment of fusiform bacteria, particularly on the rough surface. Cocci attached to those surfaces which were not heavily colonized by the fusiforms and eventually grew into and on the colonial sheets of the fusiforms. |
| Databáze: | OpenAIRE |
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