PLAG1 enhances the stemness profiles of acinar cells in normal human salivary glands in a cell type-specific manner
| Autor: | Toshiyuki Fukada, Kenji Mishima, Masayuki Azuma, Keiko Aota, Miho Ibi, Rika Yasuhara, Yuriko Goto, Tarou Irie, Hirotaka Sato, Junichi Tanaka |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pleomorphic adenoma gene 1 Ductal cells Cell Adenoma Pleomorphic Medicine (miscellaneous) Acinar Cells General Biochemistry Genetics and Molecular Biology Salivary Glands 03 medical and health sciences 0302 clinical medicine stomatognathic system Acinar cell medicine Humans Stemness Induced pluripotent stem cell General Dentistry Salivary gland Chemistry Cell growth 030206 dentistry Transfection Salivary Gland Neoplasms Cell biology DNA-Binding Proteins 030104 developmental biology medicine.anatomical_structure Tumorigenesis Salivary gland neoplasm |
| Zdroj: | Journal of oral biosciences. 62(1) |
| ISSN: | 1880-3865 |
| Popis: | Objectives: Details of the histogenesis of salivary gland tumors are largely unknown. The oncogenic role of PLAG1 in the salivary gland has been demonstrated in vivo. Herein, we demonstrate the roles of PLAG1 in the acinar and ductal cells of normal human salivary glands in an attempt to clarify the early events that occur during the histogenesis of salivary gland tumors. Methods: Normal salivary gland cells with acinar- (NS-SV-AC) and ductal- (NS-SV-DC) phenotypes were transfected with PLAG1 plasmid DNA. Subsequently, the PLAG1 overexpressed and mock cells were examined by cell proliferation, transwell migration, and salisphere formation assays. The expression levels of salivary and pluripotent stem cell markers and differentiation markers were evaluated by quantitative real-time polymerase chain reaction and immunofluorescence. Alterations in transcriptional expressions were investigated via cap analysis of gene expression with gene-enrichment and functional annotation analysis. Results: PLAG1 promoted cell proliferation and transwell migration in the acinar and ductal cells, and markedly enhanced the stemness profiles and luminal cell-like profiles in acinar cells; the stemness profiles were partially increased in the ductal cells. Conclusion: PLAG1 enhanced the stemness profiles in the acinar cells of normal human salivary glands in a cell type-specific manner. Thus, it may be involved in salivary gland tumorigenesis by increasing the stemness character of the normal salivary gland cells. |
| Databáze: | OpenAIRE |
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