Time course of global gene expression alterations in Candida albicans during infection of HeLa cells
| Autor: | Linjuan Ma, Yizhou Huang, Yibing Lan, Fan Qu, Jianhong Zhou, Juanqing Li, Jie Yan |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors 030106 microbiology Down-Regulation Gene Expression Microbiology Pathogenesis 03 medical and health sciences Gene Expression Regulation Fungal Gene expression Candida albicans Humans RNA Processing Post-Transcriptional differential gene expression Gene Oligonucleotide Array Sequence Analysis Regulation of gene expression lcsh:R5-920 biology Gene Expression Profiling Candidiasis Computational Biology General Medicine biology.organism_classification Corpus albicans infection Gene expression profiling DNA microarray lcsh:Medicine (General) Research Article HeLa Cells |
| Zdroj: | Bosnian Journal of Basic Medical Sciences, Vol 17, Iss 2 (2017) |
| Popis: | Candida albicans (C. albicans) is an opportunistic fungus that quickly adapts to various microniches. It causes candidiasis, a common fungal infection for which the pathogenic mechanism has not been elucidated yet. To explore the pathogenic mechanism of candidiasis we used several methods, including microscopic observation of morphological changes of HeLa cells and fungus, analysis of differentially expressed genes using gene chips, and a series of biological and bioinformatic analyses to explore genes that are possibly involved in the pathogenesis of C. albicans. During the C. albicans infection, significant morphological changes of the fungus were observed, and the HeLa cells were gradually destroyed. The gene chip experiments showed upregulated expression of 120 genes and downregulated expression of 178 genes. Further analysis showed that some genes may play an important role in the pathogenesis of C. albicans. Overall, morphological variation and adaptive gene expression within a particular microniche may exert important effects during C. albicans infections. |
| Databáze: | OpenAIRE |
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