Strobilanthes crispus Juice Concentrations and Anticancer Effects on DNA Damage, Apoptosis and Gene Expression in Hepatocellular Carcinoma Cells
| Autor: | Asmah Rahmat, Fauziah Othman, Abdah Md Akim, Faridah Hussin, Zolkapli Eshak, Sima Ataollahi Eshkoor |
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| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Carcinoma Hepatocellular Epidemiology DNA damage Blotting Western Apoptosis Biology Real-Time Polymerase Chain Reaction Acanthaceae Tumor Cells Cultured medicine Humans Cytotoxic T cell RNA Messenger Cell Proliferation Plant Extracts Reverse Transcriptase Polymerase Chain Reaction Cell growth Liver Neoplasms Public Health Environmental and Occupational Health Flow Cytometry medicine.disease Molecular biology Gene Expression Regulation Neoplastic Comet assay Real-time polymerase chain reaction Oncology Hepatocellular carcinoma Cancer cell Cancer research Comet Assay DNA Damage |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 16:6047-6053 |
| ISSN: | 1513-7368 |
| Popis: | Background: Hepatocellular carcinoma is one of the most common cancers worldwide. Its prevalence is increasing in many countries. Plant products can be used to protect against cancer due to natural anticancer and chemopreventive constituents. Strobilanthes crispus is one of plants with potential chemopreventive ability. Objective: This study aimed to evaluate the anticancer effects of Strobilanthes crispus juice on hepatocellular carcinoma cells. Materials and Methods: MTT assays, flow cytometry, comet assays and the reverse transcription-polymerase chain reaction (RT-PCR) were used to determine the effects of juice on DNA damage and cancer cell numbers. Results: This juice induced apoptosis after exposure of the HepG2 cell line for 72 h. High percentages of apoptotic cell death and DNA damage were seen at the juice concentrations above 0.1%. It was found that the juice was not toxic for normal cells. In addition, juice exposure increased the expression level of c-myc gene and reduced the expression level of c-fos and c-erbB2 genes in HepG2 cells. The cytotoxic effects of juice on abnormal cells were in dose dependent. Conclusions: It was concluded that the Strobilanthes crispus juice may have chemopreventive effects on hepatocellular carcinoma cells. |
| Databáze: | OpenAIRE |
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