Identification of a Novel Ligand-Receptor Pair Constitutively Activated by ras Oncogenes
| Autor: | Zhongjia Tan, Rong Zhang, Peng Liang |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Recombinant Fusion Proteins
Molecular Sequence Data Biology Ligands Biochemistry SH3 domain Cell Line Proto-Oncogene Proteins p21(ras) Anti-apoptotic Ras signalling cascade Animals Humans Genes Tumor Suppressor Amino Acid Sequence Enzyme Inhibitors Growth Substances Autocrine signalling Receptor Protein kinase A Molecular Biology Flavonoids Differential display Base Sequence Sequence Homology Amino Acid Kinase Interleukins Cell Biology Molecular biology Rats Cell biology Cell Transformation Neoplastic Genes ras Gene Expression Regulation Ras Signaling Pathway Cytokines Carrier Proteins Sequence Alignment |
| Zdroj: | Journal of Biological Chemistry. 275:24436-24443 |
| ISSN: | 0021-9258 |
| Popis: | The Ras signaling pathway is thought to control the expression of a subset of yet to be defined genes that are crucial for cell growth and differentiation. Here we have identified by differential display a novel oncogenic Ras target, mob-5, encoding a 23-kDa cytokine-like secreted protein. Mob-5 expression could be induced by oncogenic Ha-ras and Ki-ras, but not by normal ras activation. Inhibitors of both Ha-Ras and mitogen-activated protein kinase kinase completely abolished the mob-5 expression in ras transformed cells, with concomitant loss of the transformation phenotype. Using an alkaline phosphatase-tagged Mob-5 as ligand, a putative Mob-5 receptor was identified on the cell surface of oncogenic ras transformed cells. Thus, the Mob-5/Mob-5 receptor may represent a novel putative autocrine loop coordinately activated by ras oncogenes. |
| Databáze: | OpenAIRE |
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