On-chip mass spectrometric analysis in non-polar solvents by liquid beam infrared matrix-assisted laser dispersion/ionization
| Autor: | Ales Charvat, Raphael D. Urban, Benjamin Krafft, Konstantin Wink, Bernd Abel, Tillmann G. Fischer, Kirsten Zeitler, Stefan Ohla, Detlev Belder |
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| Rok vydání: | 2021 |
| Předmět: |
Aqueous solution
Materials science Atmospheric pressure Non-polar solvents Microfluidics 010401 analytical chemistry Analytical chemistry IR-MALDI 010402 general chemistry Laser 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry law.invention Condensed Matter::Soft Condensed Matter Solvent Matrix (chemical analysis) law Ionization Phase (matter) Physics::Chemical Physics Dispersion (chemistry) Reaction monitoring Research Paper |
| Zdroj: | Analytical and Bioanalytical Chemistry |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | Graphical abstract By the on-chip integration of a droplet generator in front of an emitter tip, droplets of non-polar solvents are generated in a free jet of an aqueous matrix. When an IR laser irradiates this free liquid jet consisting of water as the continuous phase and the non-polar solvent as the dispersed droplet phase, the solutes in the droplets are ionized. This ionization at atmospheric pressure enables the mass spectrometric analysis of non-polar compounds with the aid of a surrounding aqueous matrix that absorbs IR light. This works both for non-polar solvents such as n-heptane and for water non-miscible solvents like chloroform. In a proof of concept study, this approach is applied to monitor a photooxidation of N-phenyl-1,2,3,4-tetrahydroisoquinoline. By using water as an infrared absorbing matrix, analytes, dissolved in non-polar solvents from reactions carried out on a microchip, can be desorbed and ionized for investigation by mass spectrometry. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-020-03115-4. |
| Databáze: | OpenAIRE |
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