Validation of molecular biomarkers for preoperative diagnostics of human papillary thyroid carcinoma in fine needle aspirates
| Autor: | Marie-Claude Brulhart-Meynet, Mounia Heddad Masson, Patrick Meyer, Charna Dibner, Volodymyr Petrenko, Frédéric Triponez, Marc Pusztaszeri, Jacques Philippe, Samira M. Sadowski |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Thyroid nodules Pathology medicine.medical_specialty endocrine system diseases Thyroid carcinoma 03 medical and health sciences 0302 clinical medicine ddc:590 Biopsy medicine ddc:612 Prospective cohort study ddc:617 medicine.diagnostic_test business.industry medicine.disease Molecular biomarkers 030104 developmental biology 030220 oncology & carcinogenesis Biomarker (medicine) Original Article Surgery Human thyroid business Fine-needle aspirate |
| Zdroj: | Gland Surgery, Vol. 8, No Suppl 2 (2019) pp. S62-S76 |
| ISSN: | 2227-8575 2227-684X |
| DOI: | 10.21037/gs.2018.11.04 |
| Popis: | Background: Despite substantial efforts, reliable preoperative diagnostic for human thyroid malignancies in case of cytologically indeterminate nodules is still missing, resulting in high number of unnecessary thyroidectomies. In an attempt to increase precision of existing preoperative diagnostics, we aimed at validating the panel of molecular biomarkers predictive for papillary thyroid carcinoma (PTC) in preoperative fine needle aspirate (FNA) samples. Methods: In this prospective study conducted in preoperative thyroid FNA from 44 thyroid nodules, expression levels of 11 molecular biomarkers previously validated on the postoperative samples of PTCs were measured by Cell-to-CT and QuantiGene Plex methods and correlated with final diagnosis. Results: The QuantiGene Plex resulted in reliable gene expression measurements for FNA and core-needle biopsy (CNB) samples, however this method was less sensitive than pre-amplification based Cell-to-CT. Measurements conducted on the same samples by the two methods significantly correlated for most of the genes. Expression levels of TIMP1, c-MET and ARNTL were upregulated in PTC nodules as compared to benign counterparts, supporting previous post-operative studies. Strong correlation was observed between these biomarker alterations in the same samples. Within the sub-group of 15 indeterminate nodules (Bethesda II–V), TIMP1 had 100% specificity and 83% sensitivity for PTC cases. Conclusions: Assessment of TIMP1, c-MET and core-clock gene ARNTL expression levels by QuantiGene Plex assay in FNA samples holds promise as an ancillary method to the cytological preoperative diagnostics. |
| Databáze: | OpenAIRE |
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