Oligosaccharide-marker approach for qualitative and quantitative analysis of specific polysaccharide in herb formula by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry: Dendrobium officinale, a case study
| Autor: | Su-Ping Bai, Zhongzhen Zhao, Hau-Yee Fung, Li-Feng Li, Ji-Xia Zhang, Dik-Lung Ma, Li-Shuang Zhou, Tin-Long Wong, Chung-Hang Leung, Quan-Bin Han, Quan-Wei Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
Accuracy and precision
food.ingredient Oligosaccharides Mass spectrometry Polysaccharide Biochemistry Fluorescence Mass Spectrometry Analytical Chemistry chemistry.chemical_compound food Derivatization Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Molecular mass Plant Extracts Hydrolysis Organic Chemistry General Medicine Oligosaccharide Molecular Weight chemistry Herb Dendrobium Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography A. 1607:460388 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2019.460388 |
| Popis: | Qualitative and quantitative analysis of polysaccharides in herb formula remain challenge due to the limited choices of analytical methods concerning the intrinsic characteristics of large molecular mass. Herein, an oligosaccharide-marker approach was newly developed for quality assessment of polysaccharides in herbal materials, using Dendrobium officinale as a case study. This method involved partial acid hydrolysis of D. officinale polysaccharide (DOP) followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Two ABEE-labeled oligosaccharides namely, Te-Man-ABEE and Pen-Man-ABEE, were selected as chemical markers due to their high specificity in herb formula. The linear relationship between the content of these two markers and the content of DOP was then successfully established respectively. The linear relationship was further transformed to that between peak area of chemical markers and DOP content so that chemical markers were not necessary to be isolated for analysis. This linear relationship was systemically validated in terms of precision and accuracy. The results showed that these two oligosaccharide-markers presented a good linear relationship with DOP (R2 ≥ 0.997) in the range of 0.68–16.02 μg. These markers also demonstrated satisfactory precision (RSD |
| Databáze: | OpenAIRE |
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