Specific bone region localization of osteolytic versus osteoblastic lesions in a patient-derived xenograft model of bone metastatic prostate cancer
| Autor: | Robert L. Sah, Seung Chol Park, Catriona Jamieson, Amy Strasner, Nicholas A. Cacalano, Christopher J. Kane, Hiromi Kumon, Koichi Masuda, Christina C. N. Wu, Christina Jamieson, Anna A. Kulidjian, Omer A. Raheem, Michelle Muldong, Takeshi Hirata, Tomonori Yamaguchi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Urologic Diseases Pathology medicine.medical_specialty Aging Bone microenvironment Male mice FEMUR SHAFT Osteolytic lesions lcsh:RC870-923 03 medical and health sciences Prostate cancer 0302 clinical medicine Patient-derived xenograft medicine 2.1 Biological and endogenous factors Femur Aetiology Tumor xenograft Cancer business.industry Microstructural CT Prostate Cancer Bone metastasis medicine.disease lcsh:Diseases of the genitourinary system. Urology 030104 developmental biology Bone shaft Editorial Bone metastatic prostate cancer Osteoblastic lesions 030220 oncology & carcinogenesis business Bone volume Biotechnology |
| Zdroj: | Asian Journal of Urology, Vol 3, Iss 4, Pp 229-239 (2016) Asian journal of urology, vol 3, iss 4 Asian Journal of Urology |
| ISSN: | 2214-3882 |
| DOI: | 10.1016/j.ajur.2016.09.001 |
| Popis: | Objective: Bone metastasis occurs in up to 90% of men with advanced prostate cancer and leads to fractures, severe pain and therapy-resistance. Bone metastases induce a spectrum of types of bone lesions which can respond differently to therapy even within individual prostate cancer patients. Thus, the special environment of the bone makes the disease more complicated and incurable. A model in which bone lesions are reproducibly induced that mirrors the complexity seen in patients would be invaluable for pre-clinical testing of novel treatments. The microstructural changes in the femurs of mice implanted with PCSD1, a new patient-derived xenograft from a surgical prostate cancer bone metastasis specimen, were determined. Methods: Quantitative micro-computed tomography (micro-CT) and histological analyses were performed to evaluate the effects of direct injection of PCSD1 cells or media alone (Control) into the right femurs of Rag2−/−γc−/− male mice. Results: Bone lesions formed only in femurs of mice injected with PCSD1 cells. Bone volume (BV) was significantly decreased at the proximal and distal ends of the femurs (p |
| Databáze: | OpenAIRE |
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