The glycophospholipid-linked folate receptor internalizes folate without entering the clathrin-coated pit endocytic pathway
Autor: | Yun-shu Ying, Karen G. Rothberg, Richard G.W. Anderson, J F Kolhouse, Barton A. Kamen |
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Jazyk: | angličtina |
Rok vydání: | 1990 |
Předmět: |
Endosome
Glycosylphosphatidylinositols Endocytic cycle Fluorescent Antibody Technique Coated Pit Receptors Cell Surface Endosomes Endocytosis Phosphatidylinositols Clathrin KB Cells Cell Line Folic Acid Cell surface receptor Animals Humans Vitamin receptor biology Freeze Etching Folate Receptors GPI-Anchored Coated Pits Cell-Membrane Cell Biology Articles Cell biology Microscopy Electron Biochemistry Folate receptor biology.protein Glycolipids Carrier Proteins |
Zdroj: | The Journal of Cell Biology |
ISSN: | 1540-8140 0021-9525 |
Popis: | The folate receptor, also known as the membrane folate-binding protein, is maximally expressed on the surface of folate-depleted tissue culture cells and mediates the high affinity accumulation of 5-methyltetrahydrofolic acid in the cytoplasm of these cells. Recent evidence suggests that this receptor recycles during folate internalization and that it is anchored in the membrane by a glycosyl-phosphatidylinositol linkage. Using quantitative immunocytochemistry, we now show that (a) this receptor is highly clustered on the cell surface; (b) these clusters are preferentially associated with uncoated membrane invaginations rather than clathrin-coated pits; and (c) the receptor is not present in endosomes or lysosomes. This receptor appears to physically move in and out of the cell using a novel uncoated pit pathway that does not merge with the clathrin-coated pit endocytic machinery. |
Databáze: | OpenAIRE |
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