Cell proliferation and apoptosis in the olfactory epithelium of the shark Scyliorhinus canicula
| Autor: | Laura Ghigliotti, Mauro Vallarino, Lorenzo Gallus, Sara Ferrando, Chiara Gambardella, Silvia Ravera, Grazia Tagliafierro, Marino Vacchi |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Aging Cell type Cell Crypt Cartilaginous fish Apoptosis Biology Olfactory Receptor Neurons PCNA TUNEL Crypt Neurons Cellular and Molecular Neuroscience Olfactory Mucosa In Situ Nick-End Labeling medicine Animals Cell Proliferation TUNEL assay Cell growth Cell Differentiation Molecular biology Epithelium Proliferating cell nuclear antigen medicine.anatomical_structure Dogfish Models Animal biology.protein Female Olfactory epithelium Cell Division |
| Zdroj: | Journal of Chemical Neuroanatomy. 40:293-300 |
| ISSN: | 0891-0618 |
| DOI: | 10.1016/j.jchemneu.2010.08.004 |
| Popis: | To date, no study has been published on cell renewal in the olfactory epithelium of Chondrichthyes. Our work aimed at detecting proliferating cells (by Proliferating Cell Nuclear Antigen – PCNA immunohistochemistry) and apoptotic cells (by terminal uridine deoxynucleotidyl transferase nick end labeling method) in the olfactory epithelium of the shark Scyliorhinus canicula . PCNA immunoreactivity and mitotic figures were localized almost exclusively at the basal and apical thirds of the epithelial thickness. Double immunofluorescence for PCNA and OMP (a marker of mature olfactory neurons) showed that PCNA immunoreactivity is lacking in mature olfactory neurons, with the exception of crypt neurons. Crypt neurons, a cell type peculiar to fish, often showed PCNA immunoreactivity in the nucleus and may be involved in repair processes. The role of PCNA in mature crypt neurons requires further investigation to be clarified. Apoptosis was observed in sensory neurons and in basal cells. Our data highlight the presence of cell proliferation at different levels within the epithelium and the occurrence of apoptosis in both mature and proliferating cells. |
| Databáze: | OpenAIRE |
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