Autoregulation of the human cytomegalovirus major immediate-early gene
| Autor: | M F Stinski, R M Stenberg |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Genes
Viral Transcription Genetic Protein Conformation Immunology Cytomegalovirus Biology Microbiology NKX2-3 Viral Proteins Transcription (biology) Virology Chlorocebus aethiops HSPA2 Animals Humans Cloning Molecular Gene Cells Cultured HSPA9 Regulation of gene expression RNA Molecular biology Gene Expression Regulation Insect Science AKT1S1 Chromosome Deletion Research Article |
| Zdroj: | Journal of Virology. 56:676-682 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.56.3.676-682.1985 |
| Popis: | The gene coding for the human cytomegalovirus major immediate-early 72-kilodalton protein was cloned into simian virus origin of DNA replication plasmid pSVOd. Transfection of this plasmid (pSVCC2) into cells constitutively expressing the simian virus 40 T-antigen resulted in readily detectable levels of immediate-early region 1-specific RNA and protein. Partial restriction enzyme digestion of pSVCC2 was used to generate specific amino acid deletions within the 72-kilodalton protein. Mutant delta S12, which contained a deletion of 145 amino acids at the carboxy terminus of the protein, accumulated at least 10 times more immediate-early region 1 RNA than wild-type pSVCC2 did. In contrast, normal levels of delta S12-specific RNA were detected in cells cotransfected with wild-type pSVCC2. Therefore, the wild-type gene was capable of suppressing transcription of the mutant gene. Our results suggest that the wild-type major immediate-early protein of cytomegalovirus autoregulates transcription of immediate-early region 1 and that one of the regulatory domains is within the carboxy-terminal 145 amino acids of the viral protein. |
| Databáze: | OpenAIRE |
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