Desmethylbellidifolin From Gentianella acuta Ameliorate TNBS-Induced Ulcerative Colitis Through Antispasmodic Effect and Anti-Inflammation
Autor: | Yi Zhang, Yajuan Ni, Alatengchulu Da, Mengyang Liu, Jingya Ruan, Haiyang Yu, Suyile Chen, Yue Chen, Tao Wang, Yanxia Liu |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
intestine contraction Necrosis Lipopolysaccharide Gentianella acuta Inflammation Pharmacology Nitric oxide Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Pharmacology (medical) Colitis Original Research ulcerative colitis biology Chemistry desmethylbellidifolin lcsh:RM1-950 Intestine smooth muscle contraction medicine.disease Nitric oxide synthase 030104 developmental biology lcsh:Therapeutics. Pharmacology inflammation 030220 oncology & carcinogenesis biology.protein medicine.symptom |
Zdroj: | Frontiers in Pharmacology Frontiers in Pharmacology, Vol 10 (2019) |
ISSN: | 1663-9812 |
DOI: | 10.3389/fphar.2019.01104 |
Popis: | Desmethylbellidifolin (DMB) is a natural xanthone extracted from Gentianella acuta, which is used as the antidiarrhea drug in traditional Mongolian medicines. It remains unknown whether DMB can ameliorate ulcerative colitis (UC). In this study, trinitrobenzenesulfonic acid (TNBS)-induced colitis rats were treated with G. acuta extract (GAE) or DMB for 10 days. Body weight, food and water intake, rectal bleeding score, diarrhea score, and histopathological parameters were measured. Rat colon were collected to determine myeloperoxidase, nitric oxide levels, and inflammatory cytokines expression. In addition, the role of DMB on lipopolysaccharide stimulated RAW264.7 cell inflammatory response and intestine smooth muscle contraction was determined. The results showed that GAE and DMB treatment could significantly alleviate TNBS-induced UC. Colon morphological alteration, nitric oxide level, and inflammatory cytokines level, such as nitric oxide synthase, interleukin-6, tumor necrosis factor-α, and cyclooxygenase-2, were decreased. In addition, DMB attenuated lipopolysaccharide-induced nitric oxide release and proinflammatory cytokine expression in RAW264.7 cells. In isolated mice intestinal tissue, DMB also reduced the intestine smooth muscle spontaneous contraction and inhibited KCl, acetylcholine, BaCl2, or histamine-induced intestine smooth muscle active tension, while the active frequency was unaffected. Our results demonstrated that GAE and its active constituent DMB could inhibit TNBS-induced UC, reducing inflammatory response and alleviate colon muscle spasm, suggesting that DMB may be a good candidate for subsequent development as a multitargeting drug for UC treatment. |
Databáze: | OpenAIRE |
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