Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya

Autor: Sini Suomalainen, Lars Paulin, Xiaoping Zhang, Bertrand D. Eardly, Kristina Lindström, Giselle Nick, Philippe de Lajudie, Monique Gillis
Rok vydání: 1999
Předmět:
ESPECE NOUVELLE
ved/biology.organism_classification_rank.species
BACTERIE
TAXONOMIE NUMERIQUE
MICROSCOPIE ELECTRONIQUE A TRANSMISSION
Sudan
RNA
Ribosomal
16S
TECHNIQUE PCR
Phylogeny
Genetics
Gel electrophoresis
0303 health sciences
biology
NODULE RACINAIRE
FIXATION BIOLOGIQUE DE L'AZOTE
Acacia
Prosopis chilensis
Fabaceae
General Medicine
Bacterial Typing Techniques
Enzymes
Sinorhizobium
Electrophoresis
Polyacrylamide Gel
Taxonomy (biology)
Restriction fragment length polymorphism
Electrophoresis
Molecular Sequence Data
MORPHOLOGIE
TECHNIQUE RFLP
Microbiology
Numerical taxonomy
03 medical and health sciences
Bacterial Proteins
Phylogenetics
Botany
ETUDE COMPARATIVE
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
LEGUMINEUSE TROPICALE
Plants
Medicinal
030306 microbiology
ved/biology
Genes
rRNA
Sequence Analysis
DNA
biology.organism_classification
16S ribosomal RNA
Kenya
ELECTROPHORESE
ETUDE EXPERIMENTALE
MLEE.ENZYME ELECTROPHORESIS
Zdroj: University of Helsinki
ISSN: 1466-5034
1466-5026
DOI: 10.1099/00207713-49-4-1359
Popis: SDS-PAGE of total bacterial proteins was applied to the classification of 25 Sudanese and five Kenyan strains isolated from the root nodules of #Acacia senegal$ and #Prosopis chilensis$. Twenty strains were also studied by multilocus enzyme electrophoresis (MLEE) and the whole 16S rRNA gene was sequenced from two strains representing the two major clusters. These results, together with the previuously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, DNA-DNA dot-blot hybridisation, genomic fingerprinting using repetitive sequence-basec PCR, DNA base composition analysis, DNA-DNA reassociation analysis, partial sequencing of the 16S rRNA gene and RFLP analysis of the amplified 16S rRNA gene, showed that all 30 strains belong to the genus #Sinorhizobium$. Two of the strains grouped with #Sinorhizobium saheli$ and seven with #Sinorhizobium terangae$, while the rest did not cluster with any of the established species. The majority of the strains formed two phenotypically and genotypically distinct groups and we therefore propose that these strains should be classified as two new species, #Sinorhizobium arboris$ sp. nov. and #Sinorhizobium kostiense$ sp. nov. (Résumé d'auteur)
Databáze: OpenAIRE
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