Inhibition of calcification of bovine pericardium after treatment with biopolymers, E-beam irradiation and in vitro endothelization
| Autor: | Andrea C.D. Rodas, Olga Z. Higa, Roberta Polak, Reinaldo Giudici, Dennis L. Chicoma, Marisa Masumi Beppu, Ronaldo Nogueira de Moraes Pitombo |
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| Rok vydání: | 2013 |
| Předmět: |
Materials science
Simulated body fluid Fibroin Electrons Bioengineering Spectrum Analysis Raman Umbilical vein Biomaterials Calcification Physiologic Human Umbilical Vein Endothelial Cells medicine Animals Humans Endothelium Chitosan QUITOSANA Spectrometry X-Ray Emission Biomaterial Anatomy medicine.disease In vitro Endothelial stem cell Membrane Mechanics of Materials cardiovascular system Biophysics Cattle Fibroins Pericardium Calcification |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2012.08.009 |
| Popis: | This work has investigated the in vitro calcification of bovine pericardium (BP) treated with chitosan (C), silk fibroin (SF) and electron beam irradiation after its endothelization in vitro. For this purpose, freeze-dried BP membranes treated with mixtures of C and SF (1:3, 1:1 and 3:1) and then irradiated by electron beam irradiation were seeded with human umbilical vein endothelial cells (HUVEC) in vitro. After 3 weeks of cultivation these membranes were submitted to in vitro calcification tests using simulated body fluid as the calcifying agent. Control membranes were also studied (without endothelial cells exposure). The results have shown that the membrane compatibility with HUVECs in vitro prevent such biomaterial from calcifying, showing a potential application in biomaterial area, such as cardiac valves and repair patches. |
| Databáze: | OpenAIRE |
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