Polymorphic variability in the exon 19 of the RB1 gene and its flanking intronic sequences in HPV16-associated precancerous lesions in the Greek population
| Autor: | Constantina Gartzonika, Christine Kottaridi, Z. Kyriakopoulou, George D. Moschonas, Panayotis Markoulatos, T.G. Dimitriou, M. Daskou, D. Tsakogiannis, Z. Stylianidou |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Genotype Sequence analysis Ubiquitin-Protein Ligases Uterine Cervical Neoplasms Single-nucleotide polymorphism Biology Microbiology Polymerase Chain Reaction Evolution Molecular 03 medical and health sciences Exon medicine Biomarkers Tumor Humans Prospective Studies Torticollis Genetics Cervical cancer Human papillomavirus 16 Polymorphism Genetic Greece Papillomavirus Infections Intron Bayes Theorem General Medicine Exons Amplicon medicine.disease Uterine Cervical Dysplasia Introns Squamous intraepithelial lesion Retinoblastoma Binding Proteins 030104 developmental biology Dysplasia Case-Control Studies DNA Viral Female Precancerous Conditions Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of medical microbiology. 67(11) |
| ISSN: | 1473-5644 |
| Popis: | Purpose. The tumour suppressor protein RB plays a decisive role in negative control of the cell cycle, inhibiting tumour development. The present analysis investigated the prevalence of the nucleotide polymorphism A153104G, which is located at intron 18 of the RB1 gene, and investigated the impact of the polymorphic variability in the exon 19 and its flanking intronic sequences on the severity of cervical disease in HPV16-positive Greek women. Methodology. The nucleotide polymorphism A153104G was detected by PCR-RFLP assay, while the amplicons were further subjected to cloning and sequencing. Moreover, molecular evolutionary analysis was performed using the maximum-likelihood (ML) and empirical Bayesian (EB) methods in order to evaluate the selective pressure acting on exon 19 of the RB1 gene. Results/Key findings. The A153104G nucleotide polymorphism was only detected in one control case. Moreover, sequence analysis of the amplicons revealed that the polymorphic variability in the RB1 gene increased with the severity of the cervical dysplasia. The link between the observed polymorphic variability and the progress of cervical disease was reflected in the molecular evolutionary analysis that was performed on the exon 19 of the RB1 gene, since negative selective pressure was acting upon exon 19 in the control and low-grade squamous intraepithelial lesion (LSIL) cervical samples, while positive selective pressure was acting upon exon 19 in the high-grade squamous intraepithelial lesion (HSIL) specimens. Conclusions. The A153104G nucleotide polymorphism did not emerge as a potential biomarker for the development of precancerous lesions in the Greek patients, while the accumulation of sequence variations in RB1 gene might influence patients’ susceptibility towards the progression of cervical neoplasia. |
| Databáze: | OpenAIRE |
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