Direct infusion mass spectrometry as a fingerprint of protein-binding media used in works of art
| Autor: | Ernesto F. Simó-Alfonso, Juan Peris-Vicente, J. V. Gimeno Adelantado, M.T. Doménech Carbó |
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| Rok vydání: | 2005 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Resolution (mass spectrometry) Protein mass spectrometry Swine Mass spectrometry Peptide Mapping Sensitivity and Specificity Sample preparation in mass spectrometry Analytical Chemistry chemistry.chemical_compound Adhesives Animals Amino Acids Derivatization Spectroscopy chemistry.chemical_classification Chromatography Hydrolysis Organic Chemistry Proteins Reproducibility of Results Amino acid chemistry Cattle Paintings Acid hydrolysis Protein Binding |
| Zdroj: | Rapid Communications in Mass Spectrometry. 19:3463-3467 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.2217 |
| Popis: | A direct infusion mass spectrometry method for the characterization of proteinaceous glues from binding media used in pictorial works of art prior to conservation or restoration treatment is proposed. Amino acids are released by acid hydrolysis and dissolved in a mixture of acidic water and ethanol. This mixture is directly infused into a mass spectrometer without any derivatization. The mass spectrometer is operated in positive ion electrospray mode (ESI-MS) to yield [M+H]+ ions for the amino acids. Relative amounts of each amino acid are calculated for each protein (beef and porcine gelatines, albumin, casein and egg). The analyzed proteins were satisfactorily distinguished. The method is easy and fast, and shows good sensitivity and resolution. The proposed method has been successfully applied to artistic samples from items of the cultural heritage of Valencia (Spain). Copyright © 2005 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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