Lactobacillus plantarum and Bifidobacterium bifidum alleviate dry eye in mice with exorbital lacrimal gland excision by modulating gut inflammation and microbiota
| Autor: | Young-Hoo Son, Soo-Won Yun, Dong Yun Lee, Myung Joo Han, Yoon-Jung Shin, Dong-Hyun Kim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Conjunctiva Population ved/biology.organism_classification_rank.species Lacrimal gland Gut flora Microbiology Cornea Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Tear secretion education Bacteroidaceae Inflammation Mice Inbred BALB C Goblet cell education.field_of_study Bifidobacterium bifidum biology ved/biology Probiotics Lacrimal Apparatus General Medicine biology.organism_classification eye diseases Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Tears 030221 ophthalmology & optometry Cytokines Dry Eye Syndromes Lactobacillus plantarum Food Science |
| Zdroj: | Food & Function. 12:2489-2497 |
| ISSN: | 2042-650X 2042-6496 |
| Popis: | In order to understand the efficacy of probiotics against dry eye syndrome, we selected anti-inflammatory probiotics Lactobacillus plantarum NK151 and Bifidobacterium bifidum NK175, which increased the ratio of IL-10 to TNF-α expression, from the human gut bacteria collection and examined their effects on tear secretion and cornea/conjunctiva inflammation in mice with excision of the unilateral exorbital lacrimal gland and 1% atropine and 0.1% benzalkonium chloride solution (ELA)-induced dry eye. Exposure to ELA significantly reduced tear secretion in mice, assessed by the phenol red thread tear test. However, oral gavage of NK151 and/or NK175 significantly increased ELA-suppressed tear secretion, IL-10 expression, and goblet cell population and decreased the ELA-induced corneal fluorescein-staining score, IL-1β and TNF-α expression in the conjunctiva. They also suppressed ELA-induced myeloperoxidase, IL-1β, and TNF-α expression. In particular, they increased the ratio of IL-10 to TNF-α expression in the colon. Their treatments increased ELA-induced α-diversity reduction to that of the control group and partially restored ELA-shifted β-diversity to that of the control group. Oral gavage of NK151 and/or NK175 reduced ELA-induced Verrucomicrobia and Actinobacteria populations at the phylum level. Furthermore, they reduced ELA-induced Bacteroidaceae, Akkemansiaceae, and AC160630_f populations and increased ELA-suppressed Lactobacillaceae and Muribaculaceae populations at the family level. These gut bacteria populations exhibited significant correlation with the tear secretion volume. In conclusion, NK151 and/or NK175 alleviated dry eye by modulating the expression ratio of pro-inflammatory cytokines such as TNF-α and anti-inflammatory cytokines such as IL-10 and gut microbiota composition. |
| Databáze: | OpenAIRE |
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