Identification of the gene transcription repressor domain of Gli3

Autor: Piret Tiigimägi, Torben Østerlund, Piret Michelson, Robert Tsanev, Madis Metsis, Priit Kogerman
Jazyk: angličtina
Rok vydání: 2009
Předmět:
animal structures
Transcription
Genetic
β-gal
β-galactosidase
Biophysics
Kruppel-Like Transcription Factors
Repressor
TSA
trichostatin-A
Nerve Tissue Proteins
Biology
Gli3
Development
Biochemistry
Article
DMEM
Dulbecco’s modified Eagle’s medium
Histone Deacetylases
Cell Line
03 medical and health sciences
0302 clinical medicine
Structural Biology
Transcription (biology)
Zinc Finger Protein Gli3
GLI2
HDAC
histone deacetylases
Genetics
Humans
PHS
Pallister–Hall syndrome
Molecular Biology
Transcription factor
Domain
030304 developmental biology
Regulation of gene expression
0303 health sciences
YY1
Cell Biology
Hedgehog signaling pathway
Signaling
Protein Structure
Tertiary
Hh
Hedgehog
Histone Deacetylase Inhibitors
Repressor Proteins
Gene Expression Regulation
GATAD2B
030220 oncology & carcinogenesis
Ci
Cubitus interruptus
FCS
fetal calf serum
Transcription
Hedgehog
Zdroj: Febs Letters
ISSN: 1873-3468
0014-5793
Popis: Gli transcription factors are downstream targets of the Hedgehog signaling pathway. Two of the three Gli proteins harbor gene transcription repressor function in the N-terminal half. We have analyzed the sequences and identified a potential repressor domain in Gli2 and Gli3 and have tested this experimentally. Overexpression studies confirm that the N-terminal parts harbor gene repression activity and we mapped the minimal repressor to residues 106 till 236 in Gli3. Unlike other mechanisms that inhibit Gli induced gene transcription, the repressor domain identified here does not utilize Histone deacetylases (HDACs) to achieve repression, as confirmed by HDAC inhibition studies and pull-down assays. This distinguishes the identified domain from other regulatory parts with negative influence on transcription.
Databáze: OpenAIRE
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