Muscarinic acetylcholine receptor subtypes in the human iris
| Autor: | Mami Noda, Shigeru Yokoyama, Miyuki Yamamoto, Haruhiro Higashida, Kazuo Kawasaki, Nobuto Ishizaka |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
medicine.medical_specialty Iris sphincter muscle Iris Biology In Vitro Techniques Immunofluorescence Reflex Pupillary Internal medicine Muscarinic acetylcholine receptor medicine Myocyte Humans Molecular Biology Melanoma Acetylcholine receptor medicine.diagnostic_test General Neuroscience Eye Neoplasms Muscarinic acetylcholine receptor M3 Muscarinic acetylcholine receptor M2 Muscarinic acetylcholine receptor M1 Middle Aged Immunohistochemistry Receptors Muscarinic Endocrinology Microscopy Fluorescence Neurology (clinical) Fluorescein-5-isothiocyanate Developmental Biology |
| Zdroj: | Brain research. 787(2) |
| ISSN: | 0006-8993 |
| Popis: | Employing subtype-specific antisera, we measured the relative immunoreactivity of five muscarinic acetylcholine receptor (mAChR) subtype proteins (m1–m5) in the human iris. The most intensive FITC immunofluorescence was detected by the anti-m3 antibody, followed by anti-m1 and -m5 antisera, in the iris sphincter muscle cells. Only very weak fluorescence was obtained by anti-m2 and -m4 antibodies. In dilator muscle cells, weak but not consistent immunoreactivity was found by anti-m1 and -m5 antibodies. The results suggest that the m3 muscarinic receptor is the predominant subtype in sphincter muscle cells of the human iris. |
| Databáze: | OpenAIRE |
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