CD44 Expression on Epidermal Melanocytes
| Autor: | Kyle W. Herbold, Leonard M. Milstone, John G. Haggerty, Jing Zhou |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
tetradecanoyl phorbol acetate (TPA)
RNA Splicing Molecular Sequence Data keratinocyte Dermatology Biochemistry chemistry.chemical_compound Isomerism Complementary DNA medicine Humans RNA Messenger Chondroitin sulfate Molecular Biology Glycoproteins proteoglycan Base Sequence biology Melanoma Cell Membrane Alternative splicing CD44 Cell Biology medicine.disease Molecular biology carbohydrates (lipids) adhesion Hyaluronan Receptors medicine.anatomical_structure Epidermal Cells Proteoglycan chemistry Chondroitin sulfate proteoglycan Molecular Probes biology.protein Melanocytes Tetradecanoylphorbol Acetate Proteoglycans Epidermis Keratinocyte |
| Zdroj: | Journal of Investigative Dermatology. 106:1230-1235 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12348871 |
| Popis: | We examined CD44 expression on melanocytes to begin to understand what role CD44 might have in the normal behavior of melanocytes and to provide a basis for comparing CD44 expression in melanoma cells. CD44 was expressed on the entire surface of melanocytes and accentuated at the tips of dendritic processes. Two predominant forms of CD44 are expressed on cultured human foreskin melanocytes. One form has the covalent addition of chondroitin sulfate, whereas the other form has no chondroitin sulfate. Both use the hematopoietic, or CD44H, core protein. Using polymerase chain reaction primers that span the site where alternative splicing of CD44 occurs, we found only the cDNA coding CD44H. 12-O-.Tetradecanoylphorbol 13-acetate increases the size of the chondroitin sulfate chain(s) attached to CD44 but not the proportion of CD44 molecules that carry chondroitin sulfate. Ninety percent of proteoglycans on melanocytes are chondroitin sulfate proteoglycans, and the CD44 chondroitin sulfate proteoglycan represented 10% of that total. These data show that CD44H is expressed as a “part-time” chondroitin sulfate proteoglycan on normal cultured melanocytes. |
| Databáze: | OpenAIRE |
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