RNAi-mediated suppression of isoprene emission in poplar transiently impacts phenolic metabolism under high temperature and high light intensities: a transcriptomic and metabolomic analysis

Autor: Jörg-Peter Schnitzler, Andreas Kaiser, Nicolas Brüggemann, Heinz Rennenberg, Ina Zimmer, Dennis Janz, Rüdiger Hampp, Philippe Schmitt-Kopplin, Francesco Loreto, Jennifer Popko, Barbara Ehlting, Katja Behnke, Andrea Polle, Robert Hänsch, Csengele Barta
Přispěvatelé: Behnke, K, Kaiser, A, Zimmer, I, Bruggemann, N, Janz, D, Polle, A, Hampp, R, Hansch, R, Popko, J, Schmitt-Kopplin, P, Ehlting, B, Rennenberg, H, Barta, C, Loreto, F, Schnitzler, Jp
Rok vydání: 2010
Předmět:
0106 biological sciences
Hot Temperature
Light
Isoprene
H2O2
Metabolite
Environmental stress
Plant Science
01 natural sciences
chemistry.chemical_compound
Populus x canescens
Populus xcanescens
Phenolic compounds
0303 health sciences
Phenylpropanoid
food and beverages
General Medicine
Plants
Genetically Modified
Droughts
Populus
Biochemistry
Metabolome
Carbohydrate Metabolism
RNA Interference
Seasons
Down-Regulation
Biology
Genes
Plant
Photosynthesis
Models
Biological
Article
Life Sciences
Plant Pathology
Biochemistry
general
Plant Sciences
03 medical and health sciences
Hemiterpenes
Metabolomics
Phenols
Stress
Physiological
Pentanes
Butadienes
Genetics
DNA Primers
030304 developmental biology
Base Sequence
Terpenes
Gene Expression Profiling
fungi
Wild type
Hydrogen Peroxide
Metabolism
chemistry
Agronomy and Crop Science
010606 plant biology & botany
Zdroj: Plant Molecular Biology
ISSN: 1573-5028
0167-4412
Popis: In plants, isoprene plays a dual role: (a) as thermo-protective agent proposed to prevent degradation of enzymes/membrane structures involved in photosynthesis, and (b) as reactive molecule reducing abiotic oxidative stress. The present work addresses the question whether suppression of isoprene emission interferes with genome wide transcription rates and metabolite fluxes in grey poplar (Populusxcanescens) throughout the growing season. Gene expression and metabolite profiles of isoprene emitting wild type plants and RNAi-mediated non-isoprene emitting poplars were compared by using poplar Affymetrix microarrays and non-targeted FT-ICR-MS (Fourier transform ion cyclotron resonance mass spectrometry). We observed a transcriptional down-regulation of genes encoding enzymes of phenylpropanoid regulatory and biosynthetic pathways, as well as distinct metabolic down-regulation of condensed tannins and anthocyanins, in non-isoprene emitting genotypes during July, when high temperature and light intensities possibly caused transient drought stress, as indicated by stomatal closure. Under these conditions leaves of non-isoprene emitting plants accumulated hydrogen peroxide (H2O2), a signaling molecule in stress response and negative regulator of anthocyanin biosynthesis. The absence of isoprene emission under high temperature and light stress resulted transiently in a new chemo(pheno)type with suppressed production of phenolic compounds. This may compromise inducible defenses and may render non-isoprene emitting poplars more susceptible to environmental stress. Electronic supplementary material The online version of this article (doi:10.1007/s11103-010-9654-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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