| Popis: |
A protocol incorporating the concept of “bioasssay directed chemical analysis” has been developed for the analysis of polar polynuclear aromatic hydrocarbon (polar-PAH) derivatives in particulate matter samples. As an example of the protocol, results and are presented for a National Bureau of Standards (NBS) candidate standard reference diesel particulate material (SRM 1650). Good recovery of mutagenicity and mass suggests that compound decomposition/conversion was minimized. Identification of polar-PAH derivatives was undertaken for liquid chromatographic fractions of a heavy-duty diesel particulate extract that were found to contain a large portion of the total extract mutagenicity. Hydroxy-PAH, hydroxy-nitro-PAH, PAH-quinones, and nitrated heterocyclics were tentatively identified in the polar fractions of the diesel extract. The compounds of most interest at this time, because of their relatively high mutagenicity, are multifunctional nitrated-PAH (e.g., hydroxy-nitro-PAH) and nitrated-heterocyclics. Several new techniques are described that could greatly facilitate the identification of specific isomers of polar mutagens. |
