Detection of Proteins Associated with Extracellular Matrix Regulation in the Aqueous Humour of Patients with Primary Glaucoma

Autor:	Konerirajapuram Natarajan Sulochana, Wadke Vidya, Balekudaru Shantha, Karunakaran Coral, Vijaya Lingam, Ronnie George, Manish Panday, Sampath Nikhalashree
Rok vydání:	2019
Předmět:	Male Proteomics medicine.medical_specialty genetic structures Open angle glaucoma Blotting Western Cathepsin D Glaucoma Mass Spectrometry Aqueous Humor 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Western blot Ophthalmology medicine Humans Osteopontin Aged Cathepsin Extracellular Matrix Proteins biology medicine.diagnostic_test Chemistry Aqueous humour Middle Aged medicine.disease eye diseases Sensory Systems Extracellular Matrix Cystatin C Case-Control Studies 030221 ophthalmology & optometry biology.protein Female sense organs Glaucoma Angle-Closure Glaucoma Open-Angle 030217 neurology & neurosurgery Chromatography Liquid
Zdroj:	Current Eye Research. 44:1018-1025
ISSN:	1460-2202 0271-3683
DOI:	10.1080/02713683.2019.1608261
Popis:	Purpose: The protein composition of aqueous humour (AH) has held significant relevance and remains to be the prime sample in the discovery of biomarkers in glaucoma. The purpose of this study is to analyze the AH protein concentrations in primary open angle glaucoma (POAG) and primary angle closure glaucoma (PACG) and further examine the proteome changes compared to cataract control. Methods: AH was collected from 90 POAG, 72 PACG, 78 cataracts (controls) in this study. The total protein was quantified using Bradford's assay. Samples were subjected to trypsin digestion followed by liquid chromatography-mass spectrometry (LC-MS) for proteomic studies (n = 3 per group). The extracellular matrix has a major influence on the AH outflow, and the regulator proteins osteopontin (OPN), cathepsin D, and cystatin C detected by mass spectrometry are validated in AH samples by Western blot and turbidimetric immunoassay. Results: We observed a significant increase in protein levels of POAG (p = .0009); interestingly, a similar increase in PACG compared to cataract (p < .0001) and POAG (p = .02). Proteomics analysis identified 184, 190, and 299 proteins in control, POAG and PACG. OPN was increased in POAG (p = .0319) and PACG (p = .0103) compared to control. The precursor form of cathepsin D was increased in POAG and decreased in PACG, though not significant compared to control. Cystatin C was also increased in both POAG (p = .0310) and PACG (p = .0125) compared to control. Conclusion: In this study, we report for the first time that PACG cohort had higher total protein compared to controls. A qualitative comparison of proteomes revealed increased numbers of proteins identified in PACG. We assume that elevated levels of OPN and cystatin C in POAG and PACG along with altered cathepsin levels may contribute to ECM aberration in glaucoma.
Databáze:	OpenAIRE
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