Interleukin-6 mRNA expression by cortical neurons in culture: Evidence for neuronal sources of interleukin-6 production in the brain
| Autor: | Jeffrey A. Heroux, Kendra L. Burgher, Garth E. Ringheim |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Immunology Dose-Response Relationship Immunologic Mice Inbred Strains In situ hybridization Polymerase Chain Reaction Sensitivity and Specificity Proinflammatory cytokine Mice Downregulation and upregulation Animals Immunology and Allergy RNA Messenger Interleukin 6 Beta (finance) Cells Cultured In Situ Hybridization Cerebral Cortex Neurons Messenger RNA Base Sequence biology Interleukin-6 Tumor Necrosis Factor-alpha RNA Cell biology Kinetics Neurology biology.protein Tumor necrosis factor alpha Neurology (clinical) Interleukin-1 |
| Zdroj: | Journal of Neuroimmunology. 63:113-123 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/0165-5728(95)00134-4 |
| Popis: | In this study, we investigated the capacity of murine cortical neurons to express interleukin-6 (IL-6) mRNA and protein in culture. Using in situ hybridization techniques, IL-6 mRNA was localized to neuronal cells in these cultures. Moreover, IL-6 mRNA expression as measured by in situ and PCR was shown to be upregulated by the proinflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha). This was consistent with the dose and time-dependent increases in IL-6 secreted protein observed from cultures stimulated with IL-1 beta and TNF-alpha. Taken together, the data suggest that neurons are capable of participating more directly in the CNS cytokine network than previously thought and may play an important role in the inflammatory response activities in the brain. |
| Databáze: | OpenAIRE |
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