An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering
| Autor: | Janette N. Zara, Greg Asatrian, Shen Pang, Aaron W. James, David A. Stoker, Xinli Zhang, Kang Ting, Alireza Hourfar, Benjamin M. Wu, Alan Nguyen, Bruno Péault, Mirko Corselli, Silva Megerdichian, Asal Askarinam, Chia Soo, Ann M. Zhou |
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| Rok vydání: | 2012 |
| Předmět: |
Adventitia
Pathology medicine.medical_specialty Bone Regeneration Population Adipose tissue Antigens CD34 CD146 Antigen Cell Separation Biology Bone and Bones Mice Tissue engineering Tissue Engineering and Regenerative Medicine medicine Animals Humans Bone regeneration education Wound Healing education.field_of_study Tissue Engineering Tissue Scaffolds Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology General Medicine Stromal vascular fraction Adipose Tissue Leukocyte Common Antigens Wounds and Injuries Stem cell Pericytes Developmental Biology Adult stem cell |
| Zdroj: | Stem Cells Translational Medicine. 1:673-684 |
| ISSN: | 2157-6580 2157-6564 |
| DOI: | 10.5966/sctm.2012-0053 |
| Popis: | Adipose tissue is an ideal mesenchymal stem cell (MSC) source, as it is dispensable and accessible with minimal morbidity. However, the stromal vascular fraction (SVF) of adipose tissue is a heterogeneous cell population, which has disadvantages for tissue regeneration. In the present study, we prospectively purified human perivascular stem cells (PSCs) from n = 60 samples of human lipoaspirate and documented their frequency, viability, and variation with patient demographics. PSCs are a fluorescence-activated cell sorting-sorted population composed of pericytes (CD45−, CD146+, CD34−) and adventitial cells (CD45−, CD146−, CD34+), each of which we have previously reported to have properties of MSCs. Here, we found that PSCs make up, on average, 43.2% of SVF from human lipoaspirate (19.5% pericytes and 23.8% adventitial cells). These numbers were minimally changed by age, gender, or body mass index of the patient or by length of refrigerated storage time between liposuction and processing. In a previous publication, we observed that human PSCs (hPSCs) formed significantly more bone in vivo in comparison with unsorted human SVF (hSVF) in an intramuscular implantation model. We now extend this finding to a bone injury model, observing that purified hPSCs led to significantly greater healing of mouse critical-size calvarial defects than hSVF (60.9% healing as opposed to 15.4% healing at 2 weeks postoperative by microcomputed tomography analysis). These studies suggest that adipose-derived hPSCs are a new cell source for future efforts in skeletal regenerative medicine. Moreover, hPSCs are a stem cell-based therapeutic that is readily approvable by the U.S. Food and Drug Administration, with potentially increased safety, purity, identity, potency, and efficacy. |
| Databáze: | OpenAIRE |
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