Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936
| Autor: | Loren Ornelas, Paul Redmond, Simon R. Cox, Cathy Browne, Siddharthan Chandran, Emilda Gomez, Jamie Toombs, Adele M. Taylor, Lindsay Panther, Chunyan Liu, Ian J. Deary, Tara L. Spires-Jones, Karen Burr, Sally A. Cowley, Stuart J. Ritchie, Bhuvaneish T. Selvaraj, Sarah E. Harris, Tom C. Russ, Dhruv Sareen, Lee Murphy, Clive N. Svendsen, Raquel Martín-Ibáñez, James D. Cooper |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Induced Pluripotent Stem Cells Karyotype Biology LIN28 Peripheral blood mononuclear cell Article 03 medical and health sciences 0302 clinical medicine SOX2 Internal medicine medicine Cognitive decline Induced pluripotent stem cell lcsh:QH301-705.5 030304 developmental biology 0303 health sciences Cell Differentiation Cell Biology General Medicine Cellular Reprogramming 3. Good health 030104 developmental biology lcsh:Biology (General) KLF4 Ageing Leukocytes Mononuclear Reprogramming 030217 neurology & neurosurgery Developmental Biology Plasmids |
| Zdroj: | Stem Cell Research Stem Cell Research, Vol 46, Iss, Pp 101851-(2020) |
| DOI: | 10.1016/j.scr.2020.101851 |
| Popis: | Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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