Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus
| Autor: | S. Lladó Maldonado, Adama M. Sesay, Detlev Rasch, J. Krull, Nicolas Szita, Peter Panjan, Rainer Krull, Marco P.C. Marques |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Chromatography biology 010405 organic chemistry Chemistry Staphylococcus Bioengineering General Medicine Chemostat biology.organism_classification 01 natural sciences 0104 chemical sciences Dilution Cuvette Chemical kinetics Bioreactors 010608 biotechnology Bioreactor Biomass Microreactor Aeration Biotechnology Staphylococcus carnosus |
| Zdroj: | Bioprocess and biosystems engineering. 42(6) |
| ISSN: | 1615-7605 |
| Popis: | Bioreactors at the microliter scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column bioreactor made of polystyrene (working volume of 550 µL) was demonstrated. Aeration occurs through a nozzle (O ≤ 100 µm) and supports submerged whole-cell cultivation of Staphylococcus carnosus. Stationary concentrations of biomass and glucose were determined in the dilution rate regime ranging from 0.12 to 0.80 1/h with a glucose feed concentration of 1 g/L. For the first time, reaction kinetics of S. carnosus were estimated from data obtained from continuous cultivation. The maximal specific growth rate (µmax = 0.824 1/h), Monod constant (KS = 34 × 10− 3gS/L), substrate-related biomass yield coefficient (YX/S = 0.315 gCDW/gS), and maintenance coefficient (mS = 0.0035 gS/(gCDW·h)) were determined. These parameters are now available for further studies in the field of synthetic biology. |
| Databáze: | OpenAIRE |
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