Poly(ADP-ribose)polymerase activity is reduced in circulating mononuclear cells from type 2 diabetic patients
| Autor: | Gianluca Campagna, M. Sensi, Elisabetta Mandosi, Alessandra Gatti, Maria D'Erme, Leo Guidobaldi, Piera Quesada, Rosalba Cipriani, Patrizia Mancini, Pantellini F, Umberto Di Mario, Susanna Morano, Italo Tempera |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Physiology DNA repair Clinical Biochemistry Blotting Western Poly (ADP-Ribose) Polymerase-1 Inflammation Type 2 diabetes Biology Peripheral blood mononuclear cell Monocytes Internal medicine Diabetes mellitus medicine Humans Neoplastic transformation RNA Messenger Polymerase Aged Caspase 3 Reverse Transcriptase Polymerase Chain Reaction Cell Biology Middle Aged medicine.disease Blot Enzyme Activation Endocrinology Diabetes Mellitus Type 2 Microscopy Fluorescence Case-Control Studies Caspases Immunology biology.protein Female medicine.symptom Poly(ADP-ribose) Polymerases |
| Zdroj: | Journal of cellular physiology. 205(3) |
| ISSN: | 0021-9541 |
| Popis: | Poly(ADP-ribose)polymerase (PARP-1), a nuclear enzyme activated by DNA strand breaks, is involved in DNA repair, aging, inflammation, and neoplastic transformation. In diabetes, reactive oxygen and nitrogen species occurring in response to hyperglycemia cause DNA damages and PARP-1 activation. Because circulating mononuclear cells (MNCs) are involved in inflammation mechanisms, these cells were chosen as the experimental model to evaluate PARP-1 levels and activity in patients with type 2 diabetes. MNCs were isolated from 25 diabetic patients (18 M, 7 F, age, 63.5 ± 10.2 years, disease duration 17.7 ± 8.2 years) and 11 age and sex matched healthy controls. PARP-1 expression and activity were analyzed by semi-quantitative PCR, Western and activity blot, and immunofluorescence microscopy. PARP-1-mRNA expression was increased in MNCs from all diabetic patients versus controls (P |
| Databáze: | OpenAIRE |
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