Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps

Autor: Samuel Rommelaere, Bruno Lemaitre, Anna Dostalova, Mickael Poidevin
Přispěvatelé: Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Intégrité du génome et de la polarité cellulaire chez la bactérie (EQYY), Département Biologie des Génomes (DBG), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Physiology
DBG
media_common.quotation_subject
[SDV]Life Sciences [q-bio]
Mutant
Complement
Plant Science
Insect
Biology
Gram-Positive Bacteria
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Immune system
Phagocytosis
Loss of Function Mutation
Structural Biology
Immunity
Botany
Animals
Drosophila Proteins
Beauveria
Drosophila
lcsh:QH301-705.5
Ecology
Evolution
Behavior and Systematics
media_common
Serine protease
Innate immunity
Innate immune system
fungi
EQYY
Entomopathogenic fungus
Cell Biology
biology.organism_classification
Hymenoptera
Immunity
Innate
Cell biology
Drosophila melanogaster
030104 developmental biology
Parasitoid wasp
lcsh:Biology (General)
biology.protein
General Agricultural and Biological Sciences
Function (biology)
Research Article
Developmental Biology
Biotechnology
Zdroj: BMC Biology, Vol 15, Iss 1, Pp 1-16 (2017)
BMC Biology
BMC Biology, 2017, 15 (1), pp.79. ⟨10.1186/s12915-017-0408-0⟩
BMC Biology, BioMed Central, 2017, 15 (1), pp.79. ⟨10.1186/s12915-017-0408-0⟩
ISSN: 1741-7007
Popis: Background Members of the thioester-containing protein (TEP) family contribute to host defence in both insects and mammals. However, their role in the immune response of Drosophila is elusive. In this study, we address the role of TEPs in Drosophila immunity by generating a mutant fly line, referred to as TEPq Δ, lacking the four immune-inducible TEPs, TEP1, 2, 3 and 4. Results Survival analyses with TEPq Δ flies reveal the importance of these proteins in defence against entomopathogenic fungi, Gram-positive bacteria and parasitoid wasps. Our results confirm that TEPs are required for efficient phagocytosis of bacteria, notably for the two Gram-positive species tested, Staphylococcus aureus and Enterococcus faecalis. Furthermore, we show that TEPq Δ flies have reduced Toll pathway activation upon microbial infection, resulting in lower expression of antimicrobial peptide genes. Epistatic analyses suggest that TEPs function upstream or independently of the serine protease ModSP at an initial stage of Toll pathway activation. Conclusions Collectively, our study brings new insights into the role of TEPs in insect immunity. It reveals that TEPs participate in both humoral and cellular arms of immune response in Drosophila. In particular, it shows the importance of TEPs in defence against Gram-positive bacteria and entomopathogenic fungi, notably by promoting Toll pathway activation. Electronic supplementary material The online version of this article (doi:10.1186/s12915-017-0408-0) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
Externí odkaz: