Microinjection of intermediate filament proteins into living cells with and without preexisting intermediate filament network
| Autor: | Wolfram Wiegers, Peter Traub, Bernd Höner |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Neurofilament
Glial fibrillary acidic protein Microinjections Intermediate Filaments Vimentin macromolecular substances Cell Biology Biology Cell biology Cell Line Protein filament Intermediate Filament Proteins Cytoplasm Glial Fibrillary Acidic Protein biology.protein Tumor Cells Cultured Humans Cytoskeleton Intermediate filament Microinjection |
| Zdroj: | Cell biology international reports. 15(4) |
| ISSN: | 0309-1651 |
| Popis: | Human cells grown in monolayer culture were microinjected with intermediate filament subunit proteins. In fibroblasts with a preexisting vimentin network, injected porcine glial fibrillary acidic protein (GFAP) co-localized with the vimentin network within 24 hours. Phosphorylated GFAP variants were found to become dephosphorylated concomitantly with their incorporation into filamentous structures. After microinjection of either porcine GFAP or murine vimentin into human carcinoma cells lacking cytoplasmic intermediate filaments, we observed that different types of filament networks developed. Whereas vimentin was incorporated into short filaments immediately after injection, GFAP was found to aggregate into rodlike structures. This may indicate a differential filament forming ability of these intermediate filament proteins in vivo. |
| Databáze: | OpenAIRE |
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