Expression patterns of the RGS9-1 anchoring protein R9AP in the chicken and mouse suggest multiple roles in the nervous system
| Autor: | Gabor Keresztes, Stefan Heller, A. J. Hudspeth, Hideki Mutai, Hiroshi Hibino |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Nervous system
DNA Complementary Protein family Molecular Sequence Data Vesicular Transport Proteins Biology Nervous System Cellular and Molecular Neuroscience Mice Dorsal root ganglion Complementary DNA Ganglia Spinal Sequence Homology Nucleic Acid Hair Cells Auditory RGS9 medicine Animals Inner ear Photoreceptor Cells Neurons Afferent Molecular Biology Adaptor Proteins Signal Transducing Cloning Sequence Homology Amino Acid cDNA library Cell Membrane Membrane Proteins Cell Biology Molecular biology Cell biology Cell Compartmentation Protein Transport medicine.anatomical_structure sense organs SNARE Proteins Chickens RGS Proteins |
| Zdroj: | Molecular and cellular neurosciences. 24(3) |
| ISSN: | 1044-7431 |
| Popis: | In retinal photoreceptors, the duration of G protein signalling is tightly regulated by the GTPase-activating protein RGS9-1. RGS9-1 is anchored to the disk membranes of photoreceptor outer segments by association with the membrane-spanning protein R9AP. Here we report the cloning of chicken R9AP from an inner ear cDNA library and the isolation of a murine R9AP cDNA from a retinal library. In the chicken, R9AP appears to be expressed in a variety of neuronal tissues, particularly in sensory cells including inner ear hair cells, photoreceptors, and dorsal root ganglion neurons. In the mouse, R9AP is detectable predominantly in photoreceptors, but it is also weakly expressed in other areas of the central nervous system. The expression of R9AP beyond photoreceptors led us to examine potential alternative roles for R9AP besides anchoring RGS9-1 and we found sequence homology and structural similarity of the protein with members of the SNARE protein family. Expression of chicken and mouse R9AP interfered with intracellular trafficking of an indicator protein in an in vitro assay, suggesting a more active role of the protein, possibly in targeting. GTPase-activating proteins to specific membranous compartments. |
| Databáze: | OpenAIRE |
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