Cytotoxicity and Cellular Accumulation of Palladium(II) Complexes of Tetracyclines
| Autor: | Elene C. Pereira-Maia, Leda Quercia Vieira, Josianne Nicácio Silveira, Flávia C. S. de Paula, Françoise Vasconcelos Botelho, Iara Rinco Silva, Wendell Guerra |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Time Factors Cell Survival Stereochemistry Cell Antineoplastic Agents Bioengineering Biochemistry Inhibitory Concentration 50 Mice Organometallic Compounds medicine Animals Humans Cytotoxic T cell Cytotoxicity Molecular Biology Incubation Doxycycline Dose-Response Relationship Drug Chemistry General Chemistry General Medicine Mice Inbred C57BL medicine.anatomical_structure Tetracyclines Cancer cell Macrophages Peritoneal Molecular Medicine Female K562 Cells Palladium Intracellular K562 cells Nuclear chemistry medicine.drug |
| Zdroj: | Chemistry & Biodiversity. 5:2124-2130 |
| ISSN: | 1612-1880 1612-1872 |
| DOI: | 10.1002/cbdv.200890193 |
| Popis: | We studied the cytotoxic effect and the uptake of Pd(II) complexes of doxycycline (Dox), [Pd(Dox)Cl2], and tetracycline (Tc), [Pd(Tc)Cl2], in chronic myelogenous leukemia cells. The effect of the compounds on macrophage viability was also investigated. Compound 1 is more effective than compound 2 in inhibiting the growth of K562 cells with the IC(50) values of 14.44 and 34.54 microM, respectively. There is a good correlation between cell-growth inhibition and intracellular metal concentrations, determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES). Incubation of the cells with equitoxic concentrations of both compounds yields approximately the same intracellular Pd concentration. At the IC(50) doses, intracellular concentration is ca. 33 x 10(-16) mol/cell for both compounds 1 and 2. This suggests that more [Pd(Tc)Cl2] is needed to produce a cytotoxic effect, because it enters cells more slowly. Both compounds up to 16 microM did not affect the viability of mouse peritoneal macrophages after a 48-h incubation. After 72 h of incubation, the IC(50) values are 22 for [Pd(Dox)Cl2] and 40 microM for [Pd(Tc)Cl(2)]. Therefore, the cytotoxic effect in cancer cells exhibited by both compounds is higher than their effect in macrophages. |
| Databáze: | OpenAIRE |
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