In vivo biotransformation of (poly)phenols and anthocyanins of red-fleshed apple and identification of intake biomarkers
| Autor: | Alba Macià, Silvia Yuste, María Paz Romero, Rosa-Maria Valls, Maria-José Motilva, Iziar A. Ludwig, Rosa Solà, Anna Pedret, Laura Rubió |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission, Universidad Rovira i Virgili, Universidad de Lleida, Rubió, Laura, Rubió, Laura [0000-0001-8973-2942] |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Phloretin Medicine (miscellaneous) Urine Excretion Anthocyanins 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Biotransformation TX341-641 Phenols Food science Red-fleshed apples PLC-MS/MS 030109 nutrition & dietetics Nutrition and Dietetics Nutrition. Foods and food supply Red-fleshed apple food and beverages 04 agricultural and veterinary sciences 040401 food science Phenolic compounds Bioavailability Metabolic pathway chemistry Metabolic pathways UPLC-MS/MS Glucuronide Food Science |
| Zdroj: | Journal of Functional Foods, Vol 55, Iss, Pp 146-155 (2019) Recercat. Dipósit de la Recerca de Catalunya instname Repositorio Abierto de la UdL Universitad de Lleida Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 1756-4646 |
| Popis: | The aim of this study was to investigate comprehensively the metabolic pathways and human bioavailability of anthocyanins and other (poly)phenols in an apple matrix, and to elucidate potential intake biomarkers. After the acute intake of a red-fleshed apple freeze-dried snack, plasma and urine were collected and analyzed by UPLC-MS/MS. A total of 37 phase-II and microbial phenolic metabolites were detected in plasma and urine. Among these, phloretin glucuronide, cyanidin-3-O-galactoside (plasma and urine) and peonidin-3-O-galactoside (urine) were the only metabolites detected in all the volunteers and not detected at basal conditions. The maximum urine excretion was detected at 2–4 h, and the main increase in plasma of phloretin glucuronide and cyanidin-3-O-galactoside was observed at 2 h post-intake (61.0 ± 6.82 and 10.3 ± 1.50 nM, respectively). These metabolites could be selected as the best intake biomarkers of red-fleshed apple and might be useful in human intervention studies when studying its health effects. This study was supported by the Spanish Ministry of Industry, Economy and Competitiveness through the AGL2016-76943-C2-1-R and AGL2016-76943-C2-2-R projects (co-funded by the European Social Fund, European Union); I.A.L. enjoys a post-doctoral contract (2017PMF-POST2-19) from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement and from the Universitat Rovira i Virgili (URV). S.Y. was supported by a grant from the University of Lleida. In addition, the authors are grateful to NUFRI SAT (Mollerussa, Lleida, Catalonia, Spain) for providing the red-fleshed apples. A.P. has Torres Quevedo contract (Subprograma Estatal de Incorporación, Plan Estatal de Investigación Científica y Técnica y de Innovación). |
| Databáze: | OpenAIRE |
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