Evidence from capping experiments for independence of the RT7 alloantigen and the leucocyte common antigen in the rat
| Autor: | Rasmussen Gt, Hunt Hd, David M. Lubaroff |
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| Rok vydání: | 1985 |
| Předmět: |
medicine.drug_class
Immunology Mutant Fluorescent Antibody Technique Rats Inbred WF Thymus Gland PTPRC Monoclonal antibody Epitopes Antigen Histocompatibility Antigens Rats Inbred BN Genetics medicine Animals Immunologic Capping Lymphocytes Rats Inbred BUF biology Rats Inbred Strains Molecular biology Rats Inbred F344 Rats Rats Inbred Lew Antigens Surface biology.protein Leukocyte Common Antigens Antibody |
| Zdroj: | Journal of immunogenetics. 12(4-5) |
| ISSN: | 0305-1811 |
| Popis: | SUMMARY Experiments reported here demonstrate that the RT7 alloantigen and the L-C antigen are separate and distinct structures on the surface of rat lymphocytes. The distribution of the antigens in different rat strains, including the mutant WF/fz, clearly establish the RT7 antigenic system as a polymorphic diallelic system, whereas the recognized L-C antigenic determinatn is monomorphic and present on the lymphocytes of all strains tested. These data were obtained using monoclonal antibodies to the antigens in indirect immunoffluorescence experimenta. The two antigens were shown to redistribute (cap) independently of one another on the surface of rat thymocytes. Cells that had been exposed to anti-L-C antibody and FITC-cojugated anti-Ig followed by anti-RT7.1 antibody and RITC-conjugated anti-Ig demonstrated FITC caps and RITC rings. |
| Databáze: | OpenAIRE |
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