Passive sampling of environmental DNA in aquatic environments using 3D‐printed hydroxyapatite samplers

Autor: Verdier H, Christophe A. Marquette, Agnès Bouchez, Tristan Lefébure, Helen Reveron, Solène Tadier, Laurent Gremillard, Lara Konecny-Dupré, Barthes A, Thibault Datry
Rok vydání: 2022
Předmět:
Zdroj: Molecular Ecology Resources. 22:2158-2170
ISSN: 1755-0998
1755-098X
DOI: 10.1111/1755-0998.13604
Popis: The study of environmental DNA released by aquatic organisms in their habitat offers a fast, non-invasive and sensitive approach to monitor their presence. Common eDNA sampling methods such as filtration and precipitation are time consuming, require human intervention and are not applicable to a wide range of habitats such as turbid waters and poorly-accessible environments. To circumvent these limitations, we propose to use the binding properties of minerals to create a passive eDNA sampler.We have designed 3D-printed samplers made of hydroxyapatite (HAp samplers), a mineral known for its high binding affinity with DNA. The shape and the geometry of the samplers have been designed to facilitate their handling in laboratory and field. Here we describe and test the ability of HAp samplers to recover artificial DNA and eDNA.We show that HAp samplers efficiently recover DNA and are effective even on small amounts of eDNA (By better understanding the physico-chemical interactions between DNA and the HAp sampler surface, one could improve the repeatability of the sampling process and provide an easy-to-use eDNA sampling tool for aquatic environments.
Databáze: OpenAIRE
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