Changes in the Radiation Toxicity of Human Lymphoblastic T-cell Line (Jurkat) by a Common Pesticide: Diazinon
| Autor: | Zeinab Abedian, Shabestani Monfared A, Erfan Zabihi, Sajad Borzoueisileh, Asadi J, Khoshbin Khoshnazar A, Ghasemi Sh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Medical physics. Medical radiology. Nuclear medicine
Diazinon DNA damage lcsh:R895-920 diazinon Bioengineering 010501 environmental sciences Pharmacology Biology 01 natural sciences Jurkat cells 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Radiology Nuclear Medicine and imaging 030212 general & internal medicine Radiosensitivity radiotherapy 0105 earth and related environmental sciences Radiological and Ultrasound Technology radiation tolerance jurkat cells pesticides Pesticide chemistry Cell culture Toxicity Micronucleus test Original Article |
| Zdroj: | Journal of Biomedical Physics and Engineering, Vol 10, Iss 2, Pp 147-154 (2020) Journal of Biomedical Physics & Engineering |
| ISSN: | 2251-7200 |
| Popis: | Background: Diazinon is one of the most common pesticides in the world playing a similar role to radiation and it could cause DNA breaks and genetic effects. Objective: In this study, radiosensitivity of a lymphoblastic cell line pretreated by Diazinon was investigated. Material and Methods: In this case-control study, the human lymphoblastic T-cell line was divided into 6 groups based on receiving radiation or/and Diazinon. After that, the DNA damage, in all of the groups, were counted by cytokinesis-block micronuclei assay using different indices. Results: The mean frequency of micronuclei, nuclear bridges and nuclear buds in cell groups exposed by both Diazinon and radiation were remarkably higher than the other groups which just received radiation or Diazinon alone. The interaction between radiation and Diazinon treatment was statistically significant for NBUDS index. Conclusion: The results indicated that the Diazinon contamination could affect the radiosensitivity index of cancerous cells while further molecular and in-vivo studies are needed to investigate genetic and toxic effects of Diazinon on DNA and its repair system. |
| Databáze: | OpenAIRE |
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