Phospholipid metabolism in cardiomyopathic hamster heart cells
| Autor: | Hiroshi Okamoto, Hisakazu Yasuda, Hideaki Kawaguchi, Hirofumi Sawa, Toshiyuki Kudo, Mikako Shoki, Yoshihito Sakata, Hitoshi Sano |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Cardiomyopathy
Dilated Male medicine.medical_specialty Physiology Hamster Prostacyclin 6-Ketoprostaglandin F1 alpha Biology Phosphatidylinositols Calcium in biology Catalysis chemistry.chemical_compound Norepinephrine Internal medicine Cricetinae medicine Myocyte Animals Inositol Phosphatidylinositol Phospholipids Arachidonic Acid Phospholipase C Mesocricetus Cardiomyopathy Hypertrophic Enzyme Activation Endocrinology chemistry Type C Phospholipases Arachidonic acid Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | Circulation research. 69(4) |
| ISSN: | 0009-7330 |
| Popis: | We demonstrated that the activities of phosphatidylinositide-specific phospholipase C, inositol 1,4,5-trisphosphate (IP3) kinase, and IP3 phosphatase were enhanced in cardiomyopathic hamster hearts (BIO 14.6 and BIO 53.58) in comparison to control hamsters (F1b). Release of both arachidonic acid and prostacyclin was markedly enhanced by norepinephrine in the cardiomyopathic hamsters. Phospholipase C in heart has high substrate specificity to phosphatidylinositol. IP3 production was markedly enhanced in the cardiomyopathic hamsters. We also determined the intracellular calcium concentration, which was higher in BIO 53.58 hamsters than in BIO 14.6 hamsters at 5-20 weeks of age. There was no significant difference in the intracellular calcium level between F1b and BIO 14.6 hamsters at 5 weeks of age. These results suggest that phosphatidylinositol turnover stimulated by norepinephrine may produce high intracellular calcium levels in both BIO 14.6 and BIO 53.58 myocytes. In addition, in BIO 53.58 hamsters, some mechanism such as the sarcoplasmic reticulum, which controls the intracellular calcium level, may deteriorate in function. We concluded from these results that a prolonged high intracellular calcium level may lead to the death of BIO 53.58 myocytes. |
| Databáze: | OpenAIRE |
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