Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
| Autor: | Mohammad Seyedabadi, Vahid Ramezani, Hamed Fanaei, Mohsen Nabi Meibodi, Alireza Vatanara |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Chemistry Pharmaceutical Size-exclusion chromatography Beta sheet Pharmaceutical Science Infrared spectroscopy 02 engineering and technology 030226 pharmacology & pharmacy Antibodies law.invention Excipients 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Stability law Drug Discovery Administration Inhalation Desiccation Particle Size Pharmacology chemistry.chemical_classification Cyclodextrins Chromatography Cyclodextrin Calorimetry Differential Scanning Organic Chemistry beta-Cyclodextrins Trehalose 021001 nanoscience & nanotechnology chemistry Spray drying Particle Electron microscope Powders 0210 nano-technology |
| Zdroj: | Drug development and industrial pharmacy. 43(7) |
| ISSN: | 1520-5762 |
| Popis: | Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray-dried IgG.D-optimal design was employed for both experimental design and analysis and optimization of the variables. The size and aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy and electron microscopy, respectively.Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle aerodynamic behavior, in terms of fine-particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long-term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate aerodynamic property, it efficiently decreased antibody aggregation.HPBCD is an efficient excipient for the development of inhalable protein formulations. In this regard, optimal particle property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose. |
| Databáze: | OpenAIRE |
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